The SELDI-TOF MS approach to proteomics: Protein profiling and biomarker identification
The SELDI-TOF MS approach to proteomics: Protein profiling and biomarker identification
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DOI：
10.1006/bbrc.2002.6678
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发表时间：
2002-04-05
影响因子：
3.1
通讯作者：
Felschow, D
中科院分区：
文献类型：
--
作者：
Issaq, HJ;Veenstra, TD;Felschow, D
在癌症进展的早期诊断出的患者的存活率强调了鉴定疾病生物标志物的方法。表面增强的激光解吸/电离飞行时间质谱法（Seldi-Tof MS）是一种新型生物标志物发现的方法，结合了两种强大的技术：色谱和质谱法。 Seldi-Tof MS的关键特征之一是它能够从多种生物学和临床样品中提供快速蛋白质表达谱。它已用于生物标志物鉴定以及蛋白质蛋白质的研究和蛋白-DNA相互作用。 Seldi-Tof MS的多功能性允许其在项目中使用，从鉴定前列腺，膀胱，乳腺癌和卵巢癌和阿尔茨海默氏病的潜在诊断标记到研究生物分子相互作用的研究以及变速后修饰的表征。在此MinireView中，我们讨论了Seldi-Tof MS在蛋白质生物标志物发现和分析中的应用。
The need for methods to identify disease biomarkers is underscored by the survival-rate of patients diagnosed at early stages of cancer progression. Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) is a novel approach to biomarker discovery that combines two powerful techniques: chromatography and mass spectrometry. One of the key features of SELDI-TOF MS is its ability to provide a rapid protein expression profile from a variety of biological and clinical samples. It has been used for biomarker identification as well as the study of protein-protein, and protein-DNA interaction. The versatility of SELDI-TOF MS has allowed its use in projects ranging from the identification of potential diagnostic markers for prostate, bladder, breast, and ovarian cancers and Alzheimer's disease, to the study of biomolecular interactions and the characterization of posttranslational modifications. In this minireview we discuss the application of SELDI-TOF MS to protein biomarker discovery and profiling.