Identification of parthenogenesis-inducing effector proteins in Wolbachia.
Identification of parthenogenesis-inducing effector proteins in Wolbachia.
复制标题
沃尔巴克氏体中孤雌生殖诱导效应蛋白的鉴定。
DOI：
10.1101/2023.12.01.569668
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Lindsey,AmeliaRi
中科院分区：
文献类型：
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作者：
Fricke,LauraC;Lindsey,AmeliaRi
沃尔巴克氏菌属的细菌已经进化出许多策略来操纵节肢动物的性别，包括将可能的雄性后代转化为无性繁殖的雌性。这种所谓的“孤雌生殖诱导”表型可以在一些沃尔巴克氏体菌株中发现，这些菌株感染具有单倍二倍体性别决定系统的节肢动物，包括寄生蜂。尽管微生物介导的孤雌生殖在30多年前就被发现了，但潜在的遗传机制仍然难以捉摸。 我们使用了一套基因组，计算和分子工具来识别和表征两种蛋白质，这两种蛋白质在孤雌生殖诱导Wolbachia中独特地发现，并且具有宿主相关细菌效应蛋白的强烈特征。这些推定的孤雌生殖诱导蛋白与真核蛋白结构域具有同源性，包括核孔蛋白、关键的昆虫性别决定因子Transformer和具有富含亮氨酸重复序列的真核样丝氨酸-苏氨酸激酶。此外，这些蛋白质显着影响真核细胞的生物学模型酿酒酵母。我们认为，这些蛋白是孤雌生殖诱导因子，我们的研究结果表明，这将是可能的细菌-宿主相互作用的新机制。
Bacteria in the genusWolbachiahave evolved numerous strategies to manipulate arthropod sex, including the conversion of would-be male offspring to asexually reproducing females. This so-called “parthenogenesis induction” phenotype can be found in a number ofWolbachiastrains that infect arthropods with haplodiploid sex determination systems, including parasitoid wasps. Despite the discovery of microbe-mediated parthenogenesis more than 30 yr ago, the underlying genetic mechanisms have remained elusive. We used a suite of genomic, computational, and molecular tools to identify and characterize two proteins that are uniquely found in parthenogenesis-inducingWolbachiaand have strong signatures of host-associated bacterial effector proteins. These putative parthenogenesis-inducing proteins have structural homology to eukaryotic protein domains including nucleoporins, the key insect sex determining factor Transformer, and a eukaryotic-like serine–threonine kinase with leucine-rich repeats. Furthermore, these proteins significantly impact eukaryotic cell biology in the modelSaccharomyces cerevisiae. We suggest that these proteins are parthenogenesis-inducing factors and our results indicate that this would be made possible by a novel mechanism of bacterial-host interaction.