INSULIN STIMULATION OF ADIPOSE-TISSUE LIPOPROTEIN-LIPASE - USE OF THE EUGLYCEMIC CLAMP TECHNIQUE
INSULIN STIMULATION OF ADIPOSE-TISSUE LIPOPROTEIN-LIPASE - USE OF THE EUGLYCEMIC CLAMP TECHNIQUE
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DOI：
10.1172/jci110547
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发表时间：
1982-01-01
影响因子：
15.9
通讯作者：
ECKEL, RH
中科院分区：
文献类型：
--
作者：
SADUR, CN;ECKEL, RH
在11名正常受试者中研究了胰岛素在人体脂肪组织脂蛋白脂酶活性调节中的作用，并与5名对照受试者中0.9%盐水输注的作用进行了比较。在对脂蛋白脂肪酶活性进行基底脂肪组织活组织检查后，迅速输注胰岛素以达到并维持血清水平为0.000000。70 μ U/ml，而血浆葡萄糖保持在基础浓度。血清中游离脂肪酸降至27 . ±. 3%的基础（t = 5.19 P < 0.001），甘油三酯降低至77 ± 0.001。80 min时为基础值的3%（t = 3.76，P < 0.01）。脂肪组织脂蛋白脂酶活性未能增加显着高于对照组的前3小时的研究。输注胰岛素6 h后，对脂肪组织脂蛋白脂酶有明显的刺激作用（t = 3.94，P < 0.01）。葡萄糖输注量与胰岛素对酶的影响之间没有关系。6 h时脂肪组织脂蛋白脂酶活性的升高与基础脂酶活性呈负相关（r =-0.690，P < 0.02）。胰岛素似乎刺激人体脂肪组织脂蛋白脂酶活性。与抗脂解和降低血浆甘油三酯相比，胰岛素的这种作用是延迟的。胰岛素刺激的脂肪组织脂蛋白脂肪酶活性和基础酶活性之间的反比关系表明，脂肪组织本身是脂肪酶对胰岛素反应的主要调节。
The role of insulin in the regulation of adipose tissue lipoprotein lipase activity in humans was investigated in 11 normal subjects and compared with the effects of 0.9% saline infusions in 5 control subjects. After a basal adipose tissue biopsy for lipoprotein lipase activity, insulin was rapidly infused to achieve and maintain serum levels of .apprx. 70 .mu.U/ml while plasma glucose was kept at basal concentrations. Free fatty acids in serum fell to 27 .+-. 3% of basal by 20 min (t = 5.19 P < 0.001) and triglycerides decreased to 77 .+-. 3% of basal by 80 min (t = 3.76, P < 0.01). Adipose tissue lipoprotein lipase activity failed to increase significantly above that measured in controls by the first 3 h of the study. By 6 h of the infusion a stimulatory effect of insulin on adipose tissue lipoprotein lipase was found (t = 3.94, P < 0.01). There was no relationship between the amount of glucose infused and the insulin effect on the enzyme. The increase in adipose tissue lipoprotein lipase activity at 6 h, however, was inversely related to the basal lipase activity (r = -0.690, P < 0.02). Insulin appears to stimulate adipose tissue lipoprotein lipase activity in humans. This effect of insulin is delayed when compared with antilipolysis and the fall in plasma triglyceride. The inverse relationship between insulin-stimulated adipose tissue lipoprotein lipase activity and basal enzyme activity suggests that adipose tissue itself is the main regulatory of the lipase response to insulin.