Characterization of full-length tubuliform spidroin gene from Araneus ventricosus
Characterization of full-length tubuliform spidroin gene from Araneus ventricosus
复制标题
腹腹蜘蛛全长管状蛛丝蛋白基因的表征
DOI：
10.1016/j.ijbiomac.2017.07.086
复制
发表时间：
2017
影响因子：
8.2
通讯作者：
Meng Qing
中科院分区：
文献类型：
--
作者：
Wen Rui;Liu Xiangqin;Meng Qing
圆网蜘蛛产生至少七种不同的蛋白质丝，它们具有不同的机械性能。卵壳丝是由管状腺体纺成的，用于形成卵壳的外壳。迄今为止，只有两个完整的cDNA序列已被报道的管状蛛丝蛋白（TuSp，也称为CySp的圆柱形蛛丝蛋白）。在这里，我们报告了完整的基因组DNA序列的管状蛛丝（TuSp 1）基因从圆网编织蜘蛛物种Araneus ventricosus，这是通过使用长距离PCR方法获得的。大腹园蛛TuSp 1基因的主要组成部分包含一个大的外显子（5763 bp），编码1921个氨基酸残基，由9个串联重复序列控制，两侧是保守的非重复N端和C端结构域。在大腹园蛛TuSp 1中发现的这些重复序列（每个176-155个氨基酸长）是高度保守的（在氨基酸水平上>96%相同）。
Orb-web spiders produce at least seven different protein-based silk that exhibit different mechanical properties. Egg case silk is spun by tubuliform glands and used to form the outer shell of egg case. To date, only two complete cDNA sequences have been reported for tubuliform spidroin (TuSp, also known as CySp for cylindrical spidroin). Here, we report the complete genomic DNA sequence for a tubuliform spidroin (TuSp1) gene from orb-web weaving spider speciesAraneus ventricosus, which was obtained by using a long distance PCR approach. The primary component ofAraneus ventricosusTuSp1 gene contains a single large exon (5763 bp) and encodes 1921 amino acid residues dominated by 9 tandem repeats flanked by conserved non-repetitive N- and C-terminal domains. These repeats (each 176–155 amino acids long) found inAraneus ventricosusTuSp1 are highly conserved (>96% identical at amino acid level).