Difference between hemocyanin subunits from shrimp Penaeus japonicus in anti-WSSV defense
Difference between hemocyanin subunits from shrimp Penaeus japonicus in anti-WSSV defense
复制标题
日本对虾血蓝蛋白亚基在抗 WSSV 防御中的差异
DOI：
10.1016/j.dci.2007.11.010
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Xu, Xun
中科院分区：
文献类型：
--
作者：
Lei, Kaiyu;Li, Fang;Xu, Xun
血蓝蛋白是节肢动物和软体动物的呼吸蛋白，被发现具有抵御细菌和真菌入侵的功能。在本研究中，我们从日本对虾中克隆了两个血蓝蛋白亚基基因(PjHcL和PjHcY)，证明该蛋白复合体具有延缓白斑综合征病毒(WSSV)体内感染的作用。进一步研究它们在病毒入侵过程中的转录调控表明，与PjHcY亚基不同，WSSV感染可以强烈诱导PjHcL的表达。这些发现不仅揭示了血蓝蛋白在对虾抗病毒防御中的重要作用，也表明这两个亚基在对虾天然免疫中可能存在差异。(C)2007爱思唯尔有限公司。保留所有权利。
Hemocyanin, the respiratory protein of arthropods and mollusks, was found to function against bacterial and fungal invasion. In this study, we show that this protein complex could delay the infection of white spot syndrome virus (WSSV) in vivo and cloned two hemocyanin subunits genes (PjHcL and PjHcY) from the shrimp Penaeus japonicus. Further investigation of their transcriptional regulation during viral invasion showed that in contrast to subunit PjHcY, PjHcL expression could be strongly induced by WSSV infection. These findings not only reveal an important role of hemocyanin in shrimp antiviral defense but also suggest a possible discrepancy between the two subunits in shrimp innate immunity. (c) 2007 Elsevier Ltd. All rights reserved.