Characterization of Different Forms of Kava ( Piper methysticum ) Products by UPLC-MS/MS
Characterization of Different Forms of Kava ( Piper methysticum ) Products by UPLC-MS/MS
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DOI：
10.1055/a-1708-1994
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发表时间：
2022-08-22
期刊：
影响因子：
2.7
通讯作者：
Xing, Chengguo
中科院分区：
文献类型：
--
作者：
Mamallapalli, Jessica;Kanumuri, Siva Rama Raju;Xing, Chengguo
有几种形式的卡瓦（卡瓦胡椒）产品可供人类消费，已知许多因素会影响其化学成分，从而影响其药理学特性。由于卡瓦的摄入量越来越受欢迎，其内容多样性的严格表征是先决条件，特别是由于其已知的可能导致肝毒性。为了了解商业卡瓦产品中卡瓦内酯和黄酮卡瓦素的组成多样性，我们开发了一种基于UPLC-MS/MS的分析方法来定量六种卡瓦内酯（醉椒素、二氢醉椒素、麻醉椒苦素、二氢麻醉椒苦素、羊高宁和去甲氧基羊高宁）和两种黄素醉椒素（黄素卡伐菌素A和B），并分析了它们在28种不同的卡伐产品中的含量，传统的水性悬浮液和干粉。我们的研究结果表明，在分析的卡瓦制剂之间的总的和相对丰度的分析的卡瓦内酯和flavokavains方面有很大的变化。更重要的是，产品标签中的卡法根内酯丰度与我们的实验测量值相差高达90%。因此，需要对卡瓦产品的各个卡瓦内酯和卡瓦黄素的含量进行更严格和全面的质量控制。准确的含量信息对于了解不同卡瓦产品的药理特性和安全性至关重要。
There are several forms of kava ( Piper methysticum ) products available for human consumption, and many factors are known to influence their chemical compositions and therefore their pharmacological properties. Because of the increased popularity of kava intake, a rigorous characterization of their content diversity is prerequisite, particularly due to its known potential to cause hepatotoxicity. To understand the composition diversity of kavalactones and flavokavains in commercial kava products, we developed a UPLC-MS/MS-based analytical method for the quantification of six kavalactones (kavain, dihydrokavain, methysticin, dihydromethysticin, yangonin and desmethoxyyangonin) and two flavokavains (flavokavains A and B) and analyzed their contents in 28 different kava products in the form of capsules, tinctures, traditional aqueous suspensions and dried powders. Our results demonstrated a great variation in terms of the total and relative abundance of the analyzed kavalactones and flavokavains among the analyzed kava preparations. More importantly, the kavalactone abundance in the product label could differ up to 90% from our experimental measurements. Therefore, more rigorous and comprehensive quality control of kava products is required with respect to the content of individual kavalactones and flavokavains. Accurate content information is essential to understand the pharmacological properties and safety of different kava products.