ALTERNATIVELY SPLICED P53-RNA IN TRANSFORMED AND NORMAL-CELLS OF DIFFERENT TISSUE TYPES
ALTERNATIVELY SPLICED P53-RNA IN TRANSFORMED AND NORMAL-CELLS OF DIFFERENT TISSUE TYPES
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DOI：
10.1093/nar/20.8.1979
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发表时间：
1992-04-25
影响因子：
14.9
通讯作者：
KULESZMARTIN, MF
中科院分区：
文献类型：
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作者：
HAN, KA;KULESZMARTIN, MF
抑癌基因P53的选择性剪接RNA物种含有来自内含子10的另外96个碱基，其水平大约是正常表皮细胞和癌细胞中规则剪接P53 RNA水平的25%至30%。这种选择性剪接的RNA在10T1/2成纤维细胞、小鼠肝脏和睾丸中的存在表明这种选择性剪接可能是普遍的。在10T1/2细胞中，对表皮生长因子的反应，选择性剪接的P53 RNA水平与规则剪接的P53 RNA水平协同增加。用识别P53不同表位的单抗对表皮细胞进行免疫沉淀分析表明，不同的P53蛋白可能来自这两个RNA物种。考虑到以前关于羧基末端序列在P53功能中的潜在重要性的观察，了解普遍存在的选择性剪接的P53对于未来研究P53在正常细胞和肿瘤发生中的功能是很重要的。
The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast cells, mouse liver and testis suggests that this alternative splicing may be universal. The level of alternatively spliced p53 RNA was increased coordinately with that of regularly spliced p53 in 10T1/2 cells in response to epidermal growth factor. Immunoprecipitation analysis of epidermal cells using monoclonal antibodies which recognize different epitopes of p53 suggested that distinct p53 proteins may be translated from both RNA species. Considering previous observations on the potential importance of carboxyl terminal sequences in p53 function, knowledge of the ubiquitous presence of alternatively spliced p53 is important for future studies of p53 function in normal cells and in oncogenesis.