The MTT-assay as a rapid test for cell proliferation and cell killing: application to human peripheral blood lymphocytes (PBL).
The MTT-assay as a rapid test for cell proliferation and cell killing: application to human peripheral blood lymphocytes (PBL).
复制标题
DOI：
--
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
H. Weichert;I. Blechschmidt;S. Schröder;H. Ambrosius
中科院分区：
文献类型：
--
作者：
H. Weichert;I. Blechschmidt;S. Schröder;H. Ambrosius
本文研究了MTT比色法测定人外周血淋巴细胞增殖和细胞死亡的可能性。在100，000 - 800，000个细胞/孔的范围内，发现光信号（570 nm处的OD信号）与细胞数量之间存在线性相关性。用不同浓度PHA刺激后，[3 H]胸腺嘧啶核苷掺入法与MTT法之间有很好的相关性，台盼蓝拒染法和MTT法测定柔红霉素细胞毒活性的比较也表明两种方法之间有很好的相关性。结果表明，MTT法是检测人外周血淋巴细胞增殖和死亡的一种较好的方法。该测定易于操作，可以在相对短的时间内测定大量探针，并且不需要放射性。对于有色产物的测量，可以使用普通的ELISA读数器。
The possibility to use the colorimetric MTT assay for measuring proliferation and cell death of human peripheral blood lymphocytes (PBL) was studied. In a range from 100,000-800,000 cells/well a linear correlation between the optical signal (OD signal at 570 nm) and the cell number was found. It is necessary to incubate the cells with the MTT at least 2 hours. After stimulation by different PHA concentrations a very good correlation between [3H] thymidine incorporation and MTT assay was found. A comparison of daunomycin cytotoxicity, measurement by trypan blue exclusion and MTT assay, gave also a good correlation between both methods. It can be pronounced that the MTT assay is a suitable method to measure cell proliferation and cell death of human PBL. The assay is easy to handle, a large number of probes can be assayed in a relatively short time and no radioactivity is necessary. For the measurement of the colored product a common ELISA reader can be used.