Naringin, a natural dietary compound, prevents intestinal tumorigenesis in ApcMin/+ mouse model
Naringin, a natural dietary compound, prevents intestinal tumorigenesis in ApcMin/+ mouse model
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DOI：
10.1007/s00432-015-2097-9
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发表时间：
2016-05
影响因子：
3.6
通讯作者：
Yu-sheng Zhang;Ye Li;Yan Wang;Shi-Yue Sun;Tao Jiang;Cong Li;S. Cui;Xian-Jun Qu
中科院分区：
文献类型：
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作者：
Yu-sheng Zhang;Ye Li;Yan Wang;Shi-Yue Sun;Tao Jiang;Cong Li;S. Cui;Xian-Jun Qu
目的柚皮苷是一种天然的膳食黄酮类化合物。我们的目的是评估柚苷对腺瘤性结肠息肉病多发性肠肿瘤（ApcMin/+）小鼠模型中肠肿瘤发生的影响。每天灌胃，连续12周。用乙醚杀死小鼠，并收集血液样品以评估IL-6和PGE 2的浓度。切除全部肠道，检查息肉数量。肠息肉的组织样品进行了组织病理学，免疫组织化学分析和Western blotting analysis.ResultsApcMin/+小鼠饲喂柚皮苷开发较少，较小的息肉在整个肠道。柚皮苷可预防肠道肿瘤发生，且无不良反应。组织学分析显示腺瘤性息肉中异型增生细胞和异型增生减少。其作用机制可能与抗增殖、诱导凋亡及调节GSK-3β和APC/β-catenin信号通路有关。结论柚皮苷可能通过抑制细胞增殖、诱导细胞凋亡、调节GSK-3β和APC/β-catenin信号通路以及抗炎等多种途径抑制肠道肿瘤的发生。柚皮苷是一种潜在的化学预防剂，可降低结肠癌的风险。
PurposeNaringin is a natural dietary flavonoid compound. We aimed to evaluate the effects of naringin on intestinal tumorigenesis in the adenomatous polyposis coli multiple intestinal neoplasia (ApcMin/+) mouse model.MethodsApcMin/+mice were given either naringin (150 mg/kg) or vehicle by p.o. gavage daily for 12 consecutive weeks. Mice were killed with ether, and blood samples were collected to assess the concentrations of IL-6 and PGE2. Total intestines were removed, and the number of polyps was examined. Tissue samples of intestinal polyps were subjected to the assays of histopathology, immunohistochemical analysis and Western blotting analysis.ResultsApcMin/+mice fed with naringin developed less and smaller polyps in total intestines. Naringin prevented intestinal tumorigenesis without adverse effects. Histopathologic analysis revealed the reduction of dysplastic cells and dysplasia in the adenomatous polyps. The treatments’ effects might arise from its anti-proliferation, induction of apoptosis and modulation of GSK-3β and APC/β-catenin signaling pathways. Naringin also exerted its effects on tumorigenesis through anti-chronic inflammation.ConclusionNaringin prevented intestinal tumorigenesis likely through a collection of activities including anti-proliferation, induction of apoptosis, modulation of GSK-3β and APC/β-catenin pathways and anti-inflammation. Naringin is a potential chemopreventive agent for reducing the risk of colonic cancers.