Dynamics of telomerase activity in response to acute psychological stress.
Dynamics of telomerase activity in response to acute psychological stress.
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DOI：
10.1016/j.bbi.2009.11.018
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Blackburn EH
中科院分区：
文献类型：
--
作者：
Epel ES;Lin J;Dhabhar FS;Wolkowitz OM;Puterman E;Karan L;Blackburn EH
端粒酶活性在细胞生存中起着至关重要的作用，通过延长端粒和促进细胞生长和寿命。现在有可能量化人类白细胞中端粒酶活性的低水平。低基础端粒酶活性与人的慢性应激和体外慢性糖皮质激素暴露有关。在这里，我们测试白细胞端粒酶活性是否在急性心理应激下发生变化。我们将44名老年妇女，包括22名高压力痴呆护理者和22名匹配的低压力对照组，暴露在简短的实验室心理压力下，同时检测外周血单个核细胞（PBMC）端粒酶活性的变化。在基线时，照顾者的端粒酶活性水平低于对照组，但在压力下，两组端粒酶活性相似地增加。在整个样本中，应激结束一小时后端粒酶活性增加了18% （p<0.01）。端粒酶活性的增加与单核细胞、淋巴细胞和特定T细胞类型的数量或百分比的变化无关，尽管我们不能完全排除免疫细胞再分配在端粒酶活性变化中的一些潜在贡献。端粒酶活性的增加与应激源的皮质醇增加有关。最后，对任务的心理反应（更大的威胁感知）也与对照组中端粒酶活性的增加有关。这些发现揭示了动态端粒酶活性与暴露于急性应激源之间的新关系，以及应激反应的两个经典方面-感知心理应激和神经内分泌（皮质醇）对应激源的反应。
Telomerase activity plays an essential role in cel0l survival, by lengthening telomeres and promoting cell growth and longevity. It is now possible to quantify the low levels of telomerase activity in human leukocytes. Low basal telomerase activity has been related to chronic stress in people and to chronic glucocorticoid exposure in vitro. Here we test whether leukocyte telomerase activity changes under acute psychological stress. We exposed 44 elderly women, including 22 high stress dementia caregivers and 22 matched low stress controls, to a brief laboratory psychological stressor, while examining changes in telomerase activity of peripheral blood mononuclear cells (PBMC). At baseline, caregivers had lower telomerase activity levels than controls, but during stress telomerase activity increased similarly in both groups. Across the entire sample, subsequent telomerase activity increased by 18% one hour after the end of the stressor (p<0.01). The increase in telomerase activity was independent of changes in numbers or percentages of monocytes, lymphocytes, and specific T cell types, although we cannot fully rule out some potential contribution from immune cell redistribution in the change in telomerase activity. Telomerase activity increases were associated with greater cortisol increases in response to the stressor. Lastly, psychological response to the tasks (greater threat perception) was also related to greater telomerase activity increases in controls. These findings uncover novel relationships of dynamic telomerase activity with exposure to an acute stressor, and with two classic aspects of the stress response -- perceived psychological stress and neuroendocrine (cortisol) responses to the stressor.
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影响因子：
5
作者：
COHEN, S;KAMARCK, T;MERMELSTEIN, R
通讯作者：
MERMELSTEIN, R
DOI：
10.1073/pnas.0700327104
发表时间：
2007-07-10
影响因子：
11.1
作者：
Hsu, Min;McEachern, Michael J.;Lue, Neal F.
通讯作者：
Lue, Neal F.
影响因子：
3.4
作者：
Colitz, CMH;Whittington, A;Warren, J
通讯作者：
Warren, J
影响因子：
7.6
作者：
FOLKMAN, S;LAZARUS, RS
通讯作者：
LAZARUS, RS
影响因子：
8
作者：
Anderson, CJ;Hoare, SF;Keith, WN
通讯作者：
Keith, WN