Real-Time Monitoring of Phosphorylation Kinetics with Self-Assembled Nano-oscillators
Real-Time Monitoring of Phosphorylation Kinetics with Self-Assembled Nano-oscillators
复制标题
利用自组装纳米振荡器实时监测磷酸化动力学
DOI：
10.1002/anie.201411040
复制
发表时间：
2015-02-16
影响因子：
16.6
通讯作者：
Tao, Nongjian
中科院分区：
文献类型：
--
作者：
Fang, Yimin;Chen, Shan;Tao, Nongjian
磷酸化是一种翻译后修饰，涉及许多基本的细胞过程和疾病，但难以用现有技术进行真实的实时检测。报道了利用自组装纳米振荡器在真实的时间内对磷酸化的无标记检测。每个纳米振荡器由一个金纳米粒子组成，该纳米粒子通过分子连接器连接到金表面。当纳米粒子带电时，纳米振荡器可以被电场驱动振荡，并用等离子体成像方法检测。纳米振荡器测量与附着在单个纳米颗粒上的肽的磷酸化相关的电荷变化，使我们能够在没有抗体的情况下在真实的时间内研究磷酸化的动态过程，从而确定Michaelis和催化速率常数。
Phosphorylation is a post-translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label-free detection of phosphorylation is reported in real time with self-assembled nano-oscillators. Each nano-oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano-oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano-oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.