Complement factor H gene polymorphisms and Chlamydia pneumoniae infection in age-related macular degeneration.
Complement factor H gene polymorphisms and Chlamydia pneumoniae infection in age-related macular degeneration.
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DOI：
10.1038/eye.2008.422
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Binder S
中科院分区：
文献类型：
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作者：
Haas P;Steindl K;Schmid-Kubista KE;Aggermann T;Krugluger W;Hageman GS;Binder S
研究奥地利人群（高加索人种）补体因子H基因（CFH）Y 402 H多态性与年龄相关性黄斑变性（AMD）的相关性，并确定肺炎衣原体暴露（导致高达20%的社区获得性肺炎）与AMD相关CFH风险多态性之间是否存在相关性。通过聚合酶链反应-限制性片段长度多态性分析确定75例无关AMD患者的基因型，并与75例年龄匹配的健康对照组进行比较。C.采用ELISA法（R&D）检测两组患者血清中肺炎克雷伯氏菌IgG。采用χ2检验和Logistic回归分析CFH Y 402 H基因多态性与该病的相关性。CFH Y 402 H基因型频率在AMD患者和健康对照组中差异有统计学意义AMD组TT、TC和CC各1277例，分别占22.7%、53.3%和22.7%;对照组1277例，占13.3%，P值<0.005（OR：2.920/3.811）。未发现C. pneumoniae滴度与AMD的相关性（P = 0.192），而C. pneumoniae和CFH Y 402 H多态性。我们的数据证实CFH Y 402 H多态性是奥地利人群中AMD的危险因素，在AMD患者中Y 402多态性的频率更高。前C之间没有关联。肺炎感染和诊断的AMD。
To investigate the association of the complement factor H gene (CFH) Y402H polymorphism and age-related macular degeneration (AMD) in the Austrian population (Caucasoid descent), and to determine whether there is an association between exposure to Chlamydia pneumoniae—responsible for up to 20% of community-acquired pneumoniae—and the AMD-associated CFH risk polymorphism. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism analysis in 75 unrelated AMD patients and compared with 75 healthy, age-matched control subjects. C. pneumoniae serum IgG was tested by ELISA (R&D) in both groups. The association between the CFH Y402H genetic polymorphism and the disease was examined by χ2-test and logistic regression. CFH Y402H genotype frequencies differed significantly between AMD patients and healthy controls (1277 TT, 22.7%; 1277 TC, 53.3%; and 1277 CC, 22.7% in the AMD group; 1277 TT, 48.0%; 1277 TC, 38.7%; and 1277 CC, 13.3% in the control group) showing a P-value <0.005 (OR:2.920/3.811). No association was found between a positive C. pneumoniae titre and AMD (P = 0.192), nor was any association found between C. pneumoniae and the CFH Y402H polymorphism. Our data confirm that the CFH Y402H polymorphism is a risk factor for AMD in the Austrian population with a higher frequency of the Y402 polymorphism in AMD patients. No association between preceding C. pneumoniae infection and diagnosed AMD was found.