Influence of Intrapartum Antibiotic Prophylaxis for Group B Streptococcus on Gut Microbiota in the First Month of Life
Influence of Intrapartum Antibiotic Prophylaxis for Group B Streptococcus on Gut Microbiota in the First Month of Life
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DOI：
10.1097/mpg.0000000000000928
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发表时间：
2016-02-01
影响因子：
2.9
通讯作者：
Faldella, Giacomo
中科院分区：
文献类型：
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作者：
Corvaglia, Luigi;Tonti, Giacomo;Faldella, Giacomo
目的：针对 B 族链球菌 (GBS) 的产时抗生素预防 (IAP) 对婴儿肠道细菌定植的影响尚未得到广泛研究。我们的目的是评估 IAP 对健康足月婴儿肠道微生物群的影响，并探讨喂养方式的影响。方法：母亲在妊娠晚期接受过 GBS 筛查的健康足月婴儿被分为 2 组：接受 IAP 的 GBS 阳性母亲所生的婴儿与对照组的婴儿。新生儿粪便样本在出生后 7 天和 30 天时采集；提取 DNA，并通过实时 PCR 对选定的微生物群（乳杆菌属、双歧杆菌属和脆弱拟杆菌群）进行定量。结果：总共招募了 84 对婴儿-母亲。出生后 7 天时，IAP 组的双歧杆菌计数显着较低（中位数[四分位距] 6.01 Log 菌落形成单位每克 [5.51-6.98] vs 7.80 [6.61-8.26]，P=0.000）。 30 天时的双歧杆菌计数或任何时间点的乳杆菌和脆弱芽孢杆菌计数均无差异。此外，在出生 7 天时，未接受 IAP 且仅用母乳喂养的婴儿的双歧杆菌数量较高。无论 IAP 治疗如何，纯母乳喂养的婴儿在出生后 7 天和 30 天时的乳杆菌数量均较高。结论：IAP 通过减少双歧杆菌数量来改变肠道微生物区系，而接受配方奶喂养的婴儿会进一步受到影响。这些改变是否会对健康和疾病产生长期影响需要进一步调查。
Objectives: The effect of intrapartum antibiotic prophylaxis (IAP) for group B Streptococcus (GBS) on bacterial colonization of the infant's gut has not been investigated extensively. We aimed to evaluate the effect of IAP on gut microbiota in healthy term infants, also exploring the influence of type of feeding.Methods: Healthy term infants, whose mothers had been screened for GBS in late gestation, were divided into 2 groups: infants born to GBS-positive mothers who had received IAP versus controls. Neonatal fecal samples were collected at 7 and 30 days of life; DNA was extracted, and quantification of selected microbial groups (Lactobacillus spp, Bifidobacterium spp, and Bacteroides fragilis group) was performed by real-time PCR.Results: A total of 84 infant-mother pairs were recruited. Bifidobacteria count was significantly lower in the IAP group at 7 days of life (median [interquartile range] 6.01 Log colony-forming unit per gram [5.51-6.98] vs 7.80 [6.61-8.26], P=0.000). No differences in Bifidobacteria count at 30 days or in Lactobacilli and B fragilis counts at any time point were documented. Furthermore, at 7 days of life, infants who had not received IAP and were exclusively human milk-fed had higher counts of Bifidobacteria. Regardless of IAP treatment, infants fed exclusively human milk had higher Lactobacillus spp counts both at 7 and 30 days of life.Conclusions: IAP alters gut microflora by reducing the count of Bifidobacteria, which is further affected in infants receiving formula feeding. Whether these alterations could have long-term consequences on health and disease requires further investigation.