Arabidopsis NAC domain proteins VND-INTERACTING1 and ANAC103 interact with multiple NAC domain proteins
Arabidopsis NAC domain proteins VND-INTERACTING1 and ANAC103 interact with multiple NAC domain proteins
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DOI：
10.5511/plantbiotechnology.15.0208a
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发表时间：
2015-06
影响因子：
1.6
通讯作者：
M. Yamaguchi;I. Nagahage;M. Ohtani;T. Ishikawa;H. Uchimiya;M. Kawai‐Yamada;T. Demura
中科院分区：
文献类型：
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作者：
M. Yamaguchi;I. Nagahage;M. Ohtani;T. Ishikawa;H. Uchimiya;M. Kawai‐Yamada;T. Demura
拟南芥NAM、ATAF 1/2和CUC 2（NAC）结构域转录因子VND-INTERACTING 1（VNI 1）先前在酵母双杂交筛选中被分离为与木质部导管分化的关键调节因子血管相关NAC结构域蛋白7（VND 7）相互作用的因子。在这里，我们在分子水平上表征了VNI 1及其最接近的同源物ANAC 103。VNI 1和ANAC 103在体外不仅与VND蛋白相互作用，还与其他NAC结构域蛋白（例如NAC 1和CUC 2）相互作用。瞬时表达分析表明VNI 1和ANAC 103都是转录激活因子。ANAC 103启动子活性检测维管组织，以及在毛状体，保卫细胞，和嫩叶的边缘。这些数据表明，VNI 1和ANAC 103通过调节广泛的NAC结构域转录因子的转录活性来促进各种类型细胞的分化。
The Arabidopsis thaliana NAM, ATAF1/2 and CUC2 (NAC) domain transcription factor VND-INTERACTING1 (VNI1) was previously isolated as an interacting factor of VASCULAR-RELATED NAC-DOMAIN PROTEIN7 (VND7), a key regulator of xylem vessel differentiation, in a yeast two-hybrid screening. Here, we characterized VNI1 and its closest homolog, ANAC103, at the molecular level. Both VNI1 and ANAC103 interacted in vitro not only with VND proteins but also with other NAC domain proteins, such as NAC1 and CUC2. A transient expression assay showed that both VNI1 and ANAC103 are transcriptional activators. ANAC103 promoter activity was detected in vascular tissues, as well as in the trichomes, guard cells, and margins of young leaves. These data suggest that VNI1 and ANAC103 promote the differentiation of various types of cells by modulating the transcriptional activities of a wide range of NAC domain transcription factors.