Epidemiology and clinical associations of human parechovirus respiratory infections
Epidemiology and clinical associations of human parechovirus respiratory infections
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DOI：
10.1128/jcm.01207-08
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发表时间：
2008-10-01
影响因子：
9.4
通讯作者：
Simmonds, P.
中科院分区：
文献类型：
--
作者：
Harvala, H.;Robertson, I.;Simmonds, P.
人parechov （hpev）感染在幼儿中很普遍，并与轻度胃肠炎有关，与脑膜炎和新生儿败血症有关的情况较少。为了调查这些病毒与呼吸道疾病的关系，采用高度敏感的巢式聚合酶链反应筛选了2007年1月至12月收集的大量呼吸道标本档案。以前曾用聚合酶链反应对呼吸道样本进行了8种呼吸道病毒检测，包括呼吸道合胞病毒和腺病毒。3844份标本中有34份检测到HPeV，代表2220名研究对象中的27名（1.2%）。通过直接从临床标本中扩增的VP3/VP1结进行测序，鉴定HPeV类型。该方法可以高灵敏度地扩增所有HPeV类型（1型和3 ~ 6型），并在除1例筛查阳性研究标本（25例HPeV1和8例HPeV6标本）外的所有标本中鉴定出HPeV类型。HPeV1和HPeV6的感染都是季节性的，在7月和8月频率最高，仅限于6个月至5岁的儿童。在hpev阳性标本中经常同时检测到其他呼吸道病毒，其中腺病毒同时感染的比例明显过高（37%）。大多数hpev阳性标本来自急诊科，但未发现与特定呼吸道症状或疾病相关。总之，低检出率和缺乏明确的疾病相关性表明，HPeV1和-6不是呼吸道疾病患者的主要病原体。然而，所开发的筛查和分型方法将在进一步的HPeV检测中具有价值，包括检测脑膜炎病例和其他疑似HPeV相关疾病。
Infections with human parechoviruses (HPeVs) are prevalent in young children and have been associated with mild gastroenteritis and, less frequently, with meningitis and neonatal sepsis. To investigate the involvement of these viruses in respiratory disease, a highly sensitive nested PCR was used to screen a large archive of respiratory specimens, collected between January and December 2007. Respiratory samples had previously been tested for eight respiratory viruses, including respiratory syncytial virus and adenovirus, by PCR. HPeV was detected in 34 of 3,844 specimens, representing 27 of 2,220 study subjects (1.2%). HPeV types were identified by sequencing the VP3/VP1 junction amplified by PCR directly from clinical specimens. The assay could amplify all HPeV types examined with high sensitivity (types 1 and 3 to 6) and also identified HPeV types in all but one of the screen-positive study specimens (25 HPeV1 and eight HPeV6 specimens). Infections with both HPeV1 and HPeV6 were seasonal, with highest frequencies in July and August, and restricted to children aged between 6 months and 5 years. Other respiratory viruses were frequently codetected in HPeV-positive specimens, with significant overrepresentation of adenovirus coinfections (37%). Most HPeV-positive specimens were referred from emergency departments, although no association with specific respiratory symptoms or disease was found. In summary, the low frequency of detection and lack of clear disease associations indicate that HPeV1 and -6 are not major pathogens in individuals presenting with respiratory disease. However, the screening and typing methods developed will be of value in further HPeV testing, including testing for meningitis cases and other suspected HPeV-associated disease presentations.