Immunoreactivities to Three Circadian Clock Proteins in Two Ground Crickets Suggest Interspecific Diversity of the Circadian Clock Structure
Immunoreactivities to Three Circadian Clock Proteins in Two Ground Crickets Suggest Interspecific Diversity of the Circadian Clock Structure
复制标题
两种地面蟋蟀中三种生物钟蛋白的免疫反应性表明生物钟结构的种间多样性
DOI：
10.1177/0748730405283660
复制
发表时间：
2006
影响因子：
3.5
通讯作者：
M. Takeda
中科院分区：
文献类型：
--
作者：
Qi;H. Sehadová;Naoyuki Ichihara;F. Sehnal;M. Takeda
黑条长耳蟋蟀Dianemobius nigrofasciatus和Allonemobius allardi表现出相似的昼夜节律和光周期反应，表明它们具有相似的昼夜和季节时钟。为了验证这一假设，使用Period（PER）、Doubletime（DBT）和Cryptochrome（CRY）的抗血清来可视化头神经节中的昼夜节律钟神经元。免疫组化结果显示，PER、DBT和DBT主要分布于视叶、大脑间部、前脑背外侧和食管下神经节。OL内的免疫反应细胞系统在D.黑带绦虫PI和SOG免疫反应阳性，而黑带绦虫PI和SOG免疫反应阳性。allardi。每一个D。黑带肌含有3组共表达PER-ir和DBT-ir的细胞，并将额叶髓质表面上的突起发送到内层表面，表明与复眼的功能联系。在A的OL中仅发现2对PER免疫阳性细胞（无DBT免疫阳性细胞）。allardi。两个物种的大脑中都有几组PER-ir细胞。PI中也含有DBT-ir和DBT-ir细胞，但在A. Allardi，大部分DBT-IR仅限于SOG。PI和背外侧脑中的大多数免疫反应细胞将其纤维发送到对侧心侧体和咽侧体。的接近，并在某些情况下，证明身份的PER-红外线，DBT-红外线，和DBT-红外线perikarya与假定的相互作用之间的检查时钟组件是一致的。抗原总是发现在细胞质中，并没有昼夜振荡的量进行检测。光周期，控制胚胎滞育，幼虫发育的速度，蟋蟀的翅膀长度，没有明显的影响，无论是分布或强度的免疫染色。
The closely related crickets Dianemobius nigrofasciatus and Allonemobius allardi exhibit similar circadian rhythms and photoperiodic responses, suggesting that they possess similar circadian and seasonal clocks. To verify this assumption, antisera to Period (PER), Doubletime (DBT), and Cryptochrome (CRY) were used to visualize circadian clock neurons in the cephalic ganglia. Immunoreactivities referred to as PER-ir, DBT-ir, and CRY-ir were distributed mainly in the optic lobes (OL), pars intercerebralis (PI), dorsolateral protocerebrum, and the subesophageal ganglion (SOG). A system of immunoreactive cells in the OL dominates in D. nigrofasciatus, while immunoreactivities in the PI and SOG prevail in A. allardi. Each OL of D. nigrofasciatus contains 3 groups of cells that coexpress PER-ir and DBT-ir and send processes over the frontal medulla face to the inner lamina surface, suggesting functional linkage to the compound eye. Only 2 pairs of PER-ir cells (no DBT-ir) were found in the OL of A. allardi. Several groups of PER-ir cells occur in the brain of both species. The PI also contains DBT-ir and CRY-ir cells, but in A. allardi, most of the DBT-ir is confined to the SOG. Most immunoreactive cells in the PI and in the dorsolateral brain send their fibers to the contralateral corpora cardiaca and corpora allata. The proximity and, in some cases, proven identity of the PER-ir, DBT-ir, and CRY-ir perikarya are consistent with presumed interactions between the examined clock components. The antigens were always found in the cytoplasm, and no diurnal oscillations in their amounts were detected. The photoperiod, which controls embryonic diapause, the rate of larval development, and the wing length of crickets, had no discernible effect on either distribution or the intensity of the immunostaining.