The origin and kinetics of mononuclear phagocytes.
The origin and kinetics of mononuclear phagocytes.
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单核吞噬细胞的起源和动力学。
DOI：
10.1084/jem.128.3.415
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发表时间：
1968-09-01
影响因子：
15.3
通讯作者：
Cohn, Z A
中科院分区：
文献类型：
--
作者：
van Furth, R;Cohn, Z A
小鼠单核吞噬细胞传出群体的起源和周转已被描述。单核吞噬细胞定义为能够粘附于玻璃并吞噬的单核细胞。用胸苷-3H进行的体外标记研究表明，外周血和腹腔巨噬细胞中的单核细胞不会增殖，并且在正常稳态情况下可被视为终末细胞。然而，骨髓的单核吞噬细胞似乎是快速分裂的细胞。这一结论得到了体内标记实验的支持。在胸苷-3H脉冲后24小时发现标记的骨髓单核吞噬细胞的峰。24小时后，外周血中标记的单核细胞达到峰值。从这些实验可以得出结论，骨髓中快速分裂的单核吞噬细胞，称为前单核细胞，是单核细胞的祖细胞。脾切除术后和X射线照射后的标记研究排除了其他器官作为单核细胞的主要来源。血液单核细胞和腹腔巨噬细胞的峰标记同时发生。在注射新生小牛血清诱发无菌炎症后，观察到血液中的单核细胞迅速进入腹腔。这些数据得出结论，单核细胞产生腹腔巨噬细胞。没有迹象表明单核吞噬细胞来源于淋巴细胞。在正常稳态下，单核细胞通过随机过程离开循环，半衰期为22小时。计算出单核细胞的平均血液通过时间为32小时。腹腔巨噬细胞的周转率较低，估计约为每小时0.1%。在这些研究的基础上，小鼠单核吞噬细胞的生活史被公式化为：骨髓中的前单核细胞，→外周血中的单核细胞，→组织中的巨噬细胞。
The origin and turnover of efferent populations of mouse mononuclear phagocytes has been described. Mononuclear phagocytes were defined as mononuclear cells which are able to adhere to glass and phagocytize. In vitro labeling studies with thymidine-3H showed that monocytes in the peripheral blood and peritoneal macrophages do not multiply and can be considered end cells in a normal, steady state situation. However, the mononuclear phagocytes of the bone marrow appear to be rapidly dividing cells. This conclusion was supported by in vivo labeling experiments. A peak of labeled mononuclear phagocytes of the bone marrow was found 24 hr after a pulse of thymidine-3H. This was followed, 24 hr later, by a peak of labeled monocytes in the peripheral blood. From these experiments it was concluded that the rapidly dividing mononuclear phagocytes of the bone marrow, called promonocytes, are the progenitor cells of the monocytes. Labeling studies after splenectomy and after X-irradiation excluded other organs as a major source of the monocytes. Peak labeling of both the blood monocyte and peritoneal macrophages occurred at the same time. A rapid entry of monocytes from the blood into the peritoneal cavity was observed, after a sterile inflammation was evoked by an injection of newborn calf serum. These data have led to the conclusion that monocytes give rise to peritoneal macrophages. No indications have been obtained that mononuclear phagocytes originate from lymphocytes. In the normal steady state the monocytes leave the circulation by a random process, with a half-time of 22 hr. The average blood transit time of the monocytes has been calculated to be 32 hr. The turnover rate of peritoneal macrophages was low and estimated at about 0.1% per hour. On the basis of these studies the life history of mouse mononuclear phagocytes was formulated to be: promonocytes in the bone marrow, → monocytes in the peripheral blood, → macrophages in the tissue.