Comparison of Superparamagnetic and Ultrasmall Superparamagnetic Iron Oxide Cell Labeling for Tracking Green Fluorescent Protein Gene Marker with Negative and Positive Contrast Magnetic Resonance Imaging
Comparison of Superparamagnetic and Ultrasmall Superparamagnetic Iron Oxide Cell Labeling for Tracking Green Fluorescent Protein Gene Marker with Negative and Positive Contrast Magnetic Resonance Imaging
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DOI：
10.2310/7290.2009.00008
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发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Li, Debiao
中科院分区：
文献类型：
--
作者：
Zhang, Zhuoli;Dharmakumar, Rohan;Li, Debiao
本研究的目的是探讨快速低角度正对比稳态自由进动（FLAPS）成像氧化铁纳米颗粒标记的表达绿色荧光蛋白（GFP）的细胞的可行性，并将其与传统的负对比技术进行比较。用20 μ g Fe/mL浓度的超顺磁性氧化铁（SPIO）和超小超顺磁性氧化铁（USPIO）纳米颗粒孵育GFP- r3230ac细胞系（GFP细胞）24小时。制备细胞样品进行铁含量分析和细胞功能评价。标记后的细胞采用FLAPS正对比成像，并与负对比T-2*加权图像进行比较。结果表明，SPIO和USPIO标记GFP细胞对细胞功能和GFP表达均无影响。标记的细胞成功地用正、负对比磁共振成像（MRI）成像。标记的细胞在FLAPS图像中表现为信号空洞周围的信号增强窄带，在T-2*加权图像中表现为信号空洞。阳性对比成像和阴性对比成像对标记的GFP细胞的可视化都有价值。对表达GFP的标记细胞进行MRI检查，有望监测基因标记物和细胞的时空迁移，从而增强对基于细胞和基因的治疗策略的理解。
The objectives of this study were to investigate the feasibility of imaging green fluorescent protein (GFP)-expressing cells labeled with iron oxide nanoparticles with the fast low-angle positive contrast steady-state free precession (FLAPS) method and to compare them with the traditional negative contrast technique. The GFP-R3230Ac cell line (GFP cell) was incubated for 24 hours using 20 mu g Fe/mL concentration of superparamagnetic iron oxide (SPIO) and ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. Cell samples were prepared for iron content analysis and cell function evaluation. The labeled cells were imaged using positive contrast with FLAPS imaging, and FLAPS images were compared with negative contrast T-2*-weighted images. The results demonstrated that SPIO and USPIO labeling of GFP cells had no effect on cell function or GFP expression. Labeled cells were successfully imaged with both positive and negative contrast magnetic resonance imaging (MRI). The labeled cells were observed as a narrow band of signal enhancement surrounding signal voids in FLAPS images and were visible as signal voids in T-2*-weighted images. Positive contrast and negative contrast imaging were both valuable for visualizing labeled GFP cells. MRI of labeled cells with GFP expression holds potential promise for monitoring the temporal and spatial migration of gene markers and cells, thereby enhancing the understanding of cell- and gene-based therapeutic strategies.