MicroRNA gene expression deregulation in human breast cancer
MicroRNA gene expression deregulation in human breast cancer
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DOI：
10.1158/0008-5472.can-05-1783
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发表时间：
2005-08-15
期刊：
影响因子：
11.2
通讯作者：
Croce, CM
中科院分区：
文献类型：
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作者：
Iorio, MV;Ferracin, M;Croce, CM
microRNAs（miRNAs）是一类通过靶向mRNA并触发翻译抑制或RNA降解来控制基因表达的小的非编码RNA。它们的异常表达可能涉及人类疾病，包括癌症。事实上，先前已经在人类慢性淋巴细胞白血病中发现了miRNA异常表达，其中miRNA特征与特定的临床生物学特征相关。在这里，我们表明，与正常乳腺组织相比，miRNA在人类乳腺癌中也异常表达。总体miRNA表达可以清楚地区分正常与癌组织，其中最显著失调的miRNA是mir-125 b、mir-145、mir-21和mir-155。结果通过微阵列和北方印迹分析证实。我们可以鉴定其表达与特定乳腺癌生物病理学特征相关的miRNAs，如雌激素和孕激素受体表达、肿瘤分期、血管浸润或增殖指数。
MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. Their aberrant expression may be involved in human diseases, including cancer. Indeed, miRNA aberrant expression has been previously found in human chronic lymphocytic leukemias, where miRNA signatures were associated with specific clinicobiological features. Here, we show that, compared with normal breast tissue, miRNAs are also aberrantly expressed in human breast cancer. The overall miRNA expression could clearly separate normal versus cancer tissues, with the most significantly deregulated miRNAs being mir-125b, mir-145, mir-21, and mir-155. Results were confirmed by microarray and Northern blot analyses. We could identify miRNAs whose expression was correlated with specific breast cancer biopathologic features, such as estrogen and progesterone receptor expression, tumor stage, vascular invasion, or proliferation index.