Increased ratio of ICOS+/PD‐1+ follicular helper T cells positively correlates with the development of human idiopathic membranous nephropathy
Increased ratio of ICOS+/PD‐1+ follicular helper T cells positively correlates with the development of human idiopathic membranous nephropathy
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DOI：
10.1111/1440-1681.12555
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发表时间：
2016-04
影响因子：
2.9
通讯作者：
Xu Shi;Zhihui Qu;Li Zhang;N. Zhang;Yong Liu;Man Li;Jinpeng Qiu;Yanfang Jiang
中科院分区：
文献类型：
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作者：
Xu Shi;Zhihui Qu;Li Zhang;N. Zhang;Yong Liu;Man Li;Jinpeng Qiu;Yanfang Jiang
为了确定人类特发性膜性肾病（IMN）中外周血滤泡辅助T（Tfh）细胞不同亚群的频率，本研究招募了39例新发IMN患者和18例年龄和性别匹配的健康对照（HC）。采用流式细胞术检测静脉血中Tfh细胞的频率，酶联免疫吸附法检测血清IL-21浓度。采用斯皮尔曼等级相关检验评价IMN临床特征与Tfh细胞之间的相关性。总体而言，IMN患者中总的、ICOS+和PD-1+ Tfh细胞的频率增加，而ICOS+/PD-1+ Tfh细胞的比例与IMN进展呈正相关。然而，根据24小时尿蛋白水平分层的IMN患者的三个亚组中血清IL-21水平升高与HC相比无统计学显著性。尽管如此，在所有IMN患者中，Tfh细胞中的细胞内IL-21通常增加，并且与IMN的发展密切相关。最后，IL-21+ Tfh细胞的频率和ICOS+/PD-1+ Tfh细胞的比例与IMN患者24 h尿蛋白估计值呈正相关。这些数据表明，Tfh细胞有助于IMN的致病性。ICOS+/PD-1+ Tfh细胞比例和IL-21+ Tfh细胞频率可作为评价IMN发展的指标。
To identify the frequencies of different subsets of peripheral blood follicular helper T (Tfh) cells in human idiopathic membranous nephropathy (IMN), 39 patients with new onset IMN and 18 age‐ and gender‐matched healthy controls (HC) were enrolled for this study. The frequency of Tfh cells in venous blood were measured by flow cytometry, while concentration of serum IL‐21 was detected by enzyme‐linked immunosorbent assay. Correlation between the clinical features of IMN and Tfh cells was assessed by Spearman's rank correlation test. Overall, the frequencies of total, ICOS+, and PD‐1+ Tfh cells were increased in IMN patients, while the ratio of ICOS+/PD‐1+ Tfh cells positively correlated with IMN progression. However, the elevated serum IL‐21 level in three subgroups of IMN patients, stratified based on 24‐h urine protein levels, was not statistically significant compared to HC. Nonetheless, intracellular IL‐21 in Tfh cells was generally increased in all IMN patients, and closely correlated with IMN development. Finally, the frequency of IL‐21+ Tfh cells and the ratio of ICOS+/PD‐1+ Tfh cells were positively correlated with the estimated 24‐h urine protein of IMN patients. The data indicated that Tfh cells contribute to the pathogenicity of IMN. The ratio of ICOS+/PD‐1+ Tfh cells and the frequency of IL‐21+ Tfh cells may be indicators for evaluating the IMN development.