Fatty acid cycling mechanism and mitochondrial uncoupling proteins
Fatty acid cycling mechanism and mitochondrial uncoupling proteins
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DOI：
10.1016/s0005-2728(98)00084-x
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发表时间：
1998-06-10
影响因子：
4.3
通讯作者：
Garlid, KD
中科院分区：
文献类型：
--
作者：
Jezek, P;Engstovà, H;Garlid, KD
我们假设脂肪酸诱导的解偶联在生物能量系统中起作用，以设定最佳效率并调节氧化磷酸化的偶联程度。解偶联是由脂肪酸循环引起的，这是由几种遗传特异性蛋白质以及在较小程度上由其他线粒体载体实现的。哺乳动物线粒体中的解偶联调节蛋白UCP-1、UCP-2和UCP-3是解偶联调节蛋白，而植物线粒体中的解偶联调节蛋白StUCP和Escherichia coli是解偶联调节蛋白，它们都属于线粒体载体基因家族。UCP-1和假设的UCP-3主要分别在棕色脂肪组织和骨骼肌中提供非颤抖性产热。记录了UCP-1、ADP和ADP/ATP载体的脂肪酸循环，并预测了UCP-2和UCP-3的脂肪酸循环。UCP-1介导一价单极阴离子（包括阴离子脂肪酸）的嘌呤核苷酸敏感性单端口。质子化脂肪酸的返回导致Hf的单端口和解偶联。UCP-2可能参与体重和能量平衡的调节、发热和抵抗活性氧的产生。已在马铃薯块茎中发现了StUCP，并在水果和玉米中进行了免疫检测，而StUCP已从马铃薯基因文库中克隆并测序。在成熟果实的合成过程的终止和呼吸跃变型的上升过程中，β-淀粉酶被认为起作用。(C)1998年Elsevier Science B.V.
We hypothesize that fatty acid-induced uncoupling serves in bioenergetic systems to set the optimum efficiency and tune the degree of coupling of oxidative phosphorylation. Uncoupling results from fatty acid cycling, enabled by several phylogenetically specialized proteins and, to a lesser extent, by other mitochondrial carriers. It is suggested that the regulated uncoupling in mammalian mitochondria is provided by uncoupling proteins UCP-1, UCP-2 and UCP-3, whereas in plant mitochondria by PUMP and StUCP, all belonging to the gene family of mitochondrial carriers. UCP-1, and hypothetically UCP-3, serve mostly to provide nonshivering thermogenesis in brown adipose tissue and skeletal muscle, respectively. Fatty acid cycling was documented for UCP-1, PUMP and ADP/ATP carrier, and is predicted also for UCP-2 and UCP-3. UCP-1 mediates a purine nucleotide-sensitive uniport of monovalent unipolar anions, including anionic fatty acids. The return of protonated fatty acid leads to Hf uniport and uncoupling. UCP-2 is probably involved in the regulation of body weight and energy balance, in fever, and defense against generation of reactive oxygen species. PUMP has been discovered in potato tubers and immunologically detected in fruits and corn, whereas StUCP has been cloned and sequenced from a potato gene library. PUMP is supposed to act in the termination of synthetic processes in mature fruits and during the climacteric respiratory rise. (C) 1998 Elsevier Science B.V.