Prevalence and subtypes of ciprofloxacin-resistant Campylobacter spp. in commercial poultry flocks before, during, and after treatment with fluoroquinolones
Prevalence and subtypes of ciprofloxacin-resistant Campylobacter spp. in commercial poultry flocks before, during, and after treatment with fluoroquinolones
复制标题
DOI：
10.1128/aac.49.2.690-698.2005
复制
发表时间：
2005-02-01
影响因子：
4.9
通讯作者：
Piddock, LJV
中科院分区：
文献类型：
--
作者：
Humphrey, TJ;Jorgensen, F;Piddock, LJV
按照兽医的指示，对五只商业肉鸡群进行了二氟沙星或恩诺沙星治疗，以治疗临床相关感染。从处理前、处理期间和处理后的个体粪便样本以及与肉鸡环境相关的样本中分离出弯曲杆菌。在预处理的四个鸡群中检测到耐环丙沙星空肠弯曲杆菌和/或大肠杆菌菌株，但它们仅占存在的弯曲杆菌的很小比例。当用氟喹诺酮类药物处理肉鸡时，观察到耐环丙沙星弯曲杆菌的比例迅速增加。在治疗期间，几乎 100% 的弯曲杆菌具有耐药性，并且在一些鸡群中，高比例的耐药菌株在治疗后持续长达 4 周。治疗前存在多种弯曲杆菌亚型。在治疗期间和治疗后，观察到物种和亚型患病率发生了相当大的变化，但没有单一的氟喹诺酮类耐药克隆成为优势。相反，耐药性大肠杆菌菌株或耐药性大肠杆菌和空肠弯曲菌菌株的混合物成为优势，而敏感的空肠弯曲菌菌株通常在治疗前占优势。出现并成为主导的耐药亚型并不总是与治疗前检测到的相同。治疗后耐药菌株的持续存在长达 4 周，对于任何旨在避免此类菌株进入食物链的策略都具有重要意义。
Five commercial broiler chicken flocks were treated with either difloxacin or enrofloxacin for a clinically relevant infection, as instructed by a veterinarian. Campylobacters were isolated from individual fecal samples and from samples associated with the broiler environment before, during, and after treatment. Ciprofloxacin-resistant Campylobacter jejuni and/or C. coli strains were detected pretreatment in four flocks, but they constituted a very small proportion of the campylobacters present. When the broilers were treated with a fluoroquinolone, a rapid increase in the proportion of ciprofloxacin-resistant campylobacters was observed. During treatment nearly 100% of campylobacters were resistant, and in some flocks a high proportion of resistant strains persisted for up to 4 weeks after treatment. Prior to treatment a variety of campylobacter subtypes were present. During and after treatment considerable changes in both species and subtype prevalence were observed, but no single fluoroquinolone-resistant clone became dominant. Instead, resistant C. coli strains or a mixture of resistant C. coli and C.jejuni strains became dominant, whereas susceptible C.jejuni strains had usually been dominant prior to treatment. The resistant subtypes which emerged and became dominant were not always the same as those detected pretreatment. The persistence of resistant strains for up to 4 weeks posttreatment has important implications for any strategy designed to avoid the introduction of such strains into the food chain.