In Vitro and In Vivo Evaluation of Antitumor Activity of Ligustrum robustum, A Chinese Herbal Tea
In Vitro and In Vivo Evaluation of Antitumor Activity of Ligustrum robustum, A Chinese Herbal Tea
复制标题
中药茶女贞抗肿瘤活性的体外和体内评价
DOI：
--
复制
发表时间：
2019
影响因子：
2.9
通讯作者：
Pei XF
中科院分区：
文献类型：
--
作者：
Zuo HJ;Liu S;Yan C;Li LM;Pei XF
目的：研究粗壮女贞水提物对肿瘤生长的影响，并探讨其可能的分子机制。研究方法：在体外研究中，使用细胞计数试剂盒-8评估人宫颈癌细胞（HeLa）、人乳腺癌细胞（MCF-7）、人前列腺癌细胞（PC-3）、人肝癌细胞（7721）和人结肠癌细胞（SW 480）的细胞活力。人肝癌细胞（7721）和人结肠癌细胞（SW 480）用细胞计数试剂盒-8评价。李斯特通过膜联蛋白V/PI染色评价粗壮霉素处理的HeLa细胞早期或晚期凋亡。用JC-1染色细胞测量线粒体膜电位。细胞核形态学检测细胞凋亡，JC-1染色检测细胞膜电位.通过基于染色质浓缩的核形态学和通过4 '，6-二脒基-2-苯基亚硝（DAPI）染色的片段化来监测细胞凋亡。Caspase-3基于通过4 '，6-二脒基-2-苯基亚硝（DAPI）染色的染色质凝聚和片段化。半胱天冬酶-3和半胱天冬酶-8活性水平通过比色测定来测量。在体内，评价L.此外，还进行了罗勃统介导的抗肿瘤作用的裸鼠异种移植研究。结果：在体外实验中，L。robustum对HeLa、MCF-7、PC-3、7721、SW 480均有毒性作用，半数抑制浓度为2-5 mg/mL（P<0.05）。此外，还检测到磷脂酰丝氨酸外化、线粒体膜电位降低、DNA断裂和caspase-3和caspase-8激活。robustum处理的Hela细胞。利用荷Hela细胞的裸鼠模型，我们发现L。粗壮藤能显著缩小肿瘤体积和瘤重（P<0.05），但对体重和重要脏器组织学损害无明显影响。腹腔注射L.结果：罗丹明能显著降低血清谷丙转氨酶和谷草转氨酶（P<0.05）。此外，在L.罗布斯塔姆处理的肿瘤组织。结论：L. robustum在体内外均能通过caspase依赖的方式诱导肿瘤细胞凋亡而抑制肿瘤细胞生长，且无明显的肝毒性和组织学损伤，这可能为L.由于其抗肿瘤活性而被用作草药茶。
Objective: To examine the effect of the aqueous extract of Ligustrum robustum on tumor growth in vitro and in vivo and explore the possible molecular mechanisms. Methods: In in vitro study, cell viabilities of human cervical carcinoma cells (HeLa), human breast cancer cells (MCF-7), human prostate cancer cells (PC-3), human hepatoma cells (7721) and human colon carcinoma cells (SW480) were evaluated with cell counting kit-8. human hepatoma cells (7721) and human colon carcinoma cells (SW480) were evaluated with cell counting kit-8. For L. robustum-treated Hela cells, early or late apoptosis were evaluated by annexin V/PI staining. Mitochondrial membrane potential was measured by staining cells with JC-1. Apoptosis was monitored by nuclear morphology membrane potential was measured by staining cells with JC-1. Apoptosis was monitored by nuclear morphology based on chromatin condensation and fragmentation by 4',6-diamidino-2-phenylinole (DAPI) staining. Caspase-3 based on chromatin condensation and fragmentation by 4',6-diamidino-2-phenylinole (DAPI) staining. Caspase-3 and -8 activity levels were measured by a colorimetric assay. In vivo In vivo, to evaluate the possible mechanism of , to evaluate the possible mechanism of L. robustum-mediated antitumor effect, nude mouse xenograft study was also conducted. Results: In in vitro study, L. robustum was found to be toxic to HeLa, MCF-7, PC-3, 7721, SW480, with an half maximal inhibitory concentration value of 2–5 mg/mL (P<0.05). Moreover, externalization of phosphatidylserine, loss of mitochondrial membrane potential, DNA fragmentation and activation of caspase-3 and -8 were detected in L. robustumtreated Hela cells. Using a nude mouse model bearing Hela xenografts, we found that L. robustum reduced tumor volume and tumor weight (P<0.05), but had no effect on body weight and histological damage of important organs. Intraperitoneal injection of L. robustum caused a signi caused a signififi cant reduction in serum aspartate transaminase cant reduction in serum aspartate transaminase and alanine transaminase levels (P<0.05). Furthermore, cleaved caspase-3-positive and terminal nucleotidyl transferase-mediated nick end labeling (TUNEL)-positive cells were observed in L. robustum-treated tumor tissues. Conclusions: L. robustum inhibits tumor cell growth both in vitro and in vivo by inducing apoptosis in a caspasedependent way without apparent hepatic toxicity and histological damage, which may offer partial scienti dependent way without apparent hepatic toxicity and histological damage, which may offer partial scientififi c support c support for the ethnopharmacological claims of L. robustum as a herbal tea for its antitumor activity.