Identification of a class of CFEM proteins containing a new conserved motif in Fusarium oxysporum
Identification of a class of CFEM proteins containing a new conserved motif in Fusarium oxysporum
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尖孢镰刀菌中一类含有新保守基序的 CFEM 蛋白的鉴定
DOI：
10.1016/j.pmpp.2014.12.001
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发表时间：
2015-01-01
影响因子：
2.7
通讯作者：
Xie, Bingyan
中科院分区：
文献类型：
--
作者：
Ling, Jian;Zeng, Feng;Xie, Bingyan
尖孢镰刀菌（Fusarium oxysporum，Fo）是一种重要的土传真菌，在世界范围内造成巨大的经济损失。真菌特异性胞外膜蛋白（CFEM）参与了一些重要的致病生理过程。迄今为止，很少有Fo CFEM蛋白质已被表征。最近发表的几个基因组的Fo使我们能够进行全基因组的比较分析的CFEM蛋白在Fo。在这项研究中，我们确定了CFEM蛋白的12个不同的Fo formae speciales（f。sp），并且对于每个Fo获得平均16个CFEM蛋白。根据结构特征将Fo CFEM蛋白分为三组（组1-3）。重要的是，我们确定了一个新的保守基序，包含约50个氨基酸（DR基序）的第1组成员。含DR基序的CFEM蛋白（CFEM_DR蛋白）在Fo中具有很高的保守性，其数量在Fo中较多。我们发现CFEM_ DR蛋白在Fo中的扩增可以归因于基因组中的片段复制。表达分析表明，CFEM_DR基因在菌丝体中的表达量高于分生孢子，在接种后3d，其在寄主根中的表达量显著增加，表明Fo CFEM_DR基因在侵染寄主过程中发挥作用。(C)2014爱思唯尔有限公司版权所有。
Fusarium oxysporum (Fo) is an important soil-borne fungus that cause huge economic losses worldwide. Fungal-specific Common in Fungal Extracellular Membrane (CFEM) proteins are known to be involved in some important physiological processes associated with pathogenicity. To date, few Fo CFEM proteins have been characterized. The recent publication of several genomes of Fo has allowed us to conduct a genome-wide comparison analysis of CFEM proteins in Fo. In this study, we identified CFEM proteins for 12 different Fo formae speciales(f. sp) and obtained an average of 16 CFEM proteins for each Fo. The Fo CFEM proteins were classified into three groups (groups 1-3) according to structural features. Importantly, we identified a new conserved motif containing about 50 amino acids (DR motif) in group 1 members. CFEM proteins containing DR motif (CFEM_DR proteins) are remarkably conserved among Fo, and their number is greater in Fo compared with other fungi. We found the expansion of CFEM_ DR proteins in Fo can be attributed to the segmental duplications in the genomes. Expression analysis revealed that CFEM_DR genes had a higher expression level in mycelium than conidia, and their expressions increased dramatically in the host roots at 3 days post inoculation, indicating that Fo CFEM_DR genes have roles while colonizing and infecting their hosts. (C) 2014 Elsevier Ltd. All rights reserved.