Onset of neonatal locomotor behavior and the mechanical development of Achilles and tail tendons
Onset of neonatal locomotor behavior and the mechanical development of Achilles and tail tendons
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DOI：
10.1016/j.jbiomech.2019.109354
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发表时间：
2019-11-11
影响因子：
2.4
通讯作者：
Schiele, Nathan R.
中科院分区：
文献类型：
--
作者：
Theodossiou, Sophia K.;Bozeman, Aimee L.;Schiele, Nathan R.
肌腱组织工程方法受到对机械负荷在正常肌腱发育中所起作用的有限认识的挑战。我们认为，随着运动行为的开始，出生后肌腱发育所经历的负荷增加会影响肌腱的形成。本研究的目的是评估出生后第1天、第5天和第10天大鼠自发负重运动的开始，并表征负重和非负重肌腱机械发育与运动的关系。运动被录像并评分，以确定在P1、P5和P10时的非负重、部分负重和完全负重的运动行为。对作为负重肌腱的跟腱和作为非负重肌腱的尾腱进行了力学评估。我们观察到，与P1和P5相比，P10大鼠的运动行为显著增加。我们还发现，跟腱的最大力、刚度、最大力时的位移和横截面积随着出生后年龄的变化而有相应的显著差异。然而，最大应力、最大应力下的应变和弹性模数保持不变。与P5组相比，P10组大鼠尾部肌腱的最大受力、最大应力、弹性模量值和刚度均显著增加。我们的结果表明，运动行为的开始可能提供了调节出生后肌腱生长的力学线索，它们在负重和非负重肌腱中的机械发育可能是不同的。进一步分析这种负荷如何影响体内发育的肌腱，可能会为未来的工程方法提供参考，这些工程方法旨在应用这种机械线索来调节体外工程化肌腱的形成。(C)2019爱思唯尔有限公司。保留所有权利。
Tendon tissue engineering approaches are challenged by a limited understanding of the role mechanical loading plays in normal tendon development. We propose that the increased loading that developing postnatal tendons experience with the onset of locomotor behavior impacts tendon formation. The objective of this study was to assess the onset of spontaneous weight-bearing locomotion in postnatal day (P) 1, 5, and 10 rats, and characterize the relationship between locomotion and the mechanical development of weight-bearing and non-weight-bearing tendons. Movement was video recorded and scored to determine non-weight-bearing, partial weight-bearing, and full weight-bearing locomotor behavior at P1, P5, and P10. Achilles tendons, as weight-bearing tendons, and tail tendons, as non-weight-bearing tendons, were mechanically evaluated. We observed a significant increase in locomotor behavior in P10 rats, compared to P1 and P5. We also found corresponding significant differences in the maximum force, stiffness, displacement at maximum force, and cross-sectional area in Achilles tendons, as a function of postnatal age. However, the maximum stress, strain at maximum stress, and elastic modulus remained constant. Tail tendons of P10 rats had significantly higher maximum force, maximum stress, elastic modulus, and stiffness compared to P5. Our results suggest that the onset of locomotor behavior may be providing the mechanical cues regulating postnatal tendon growth, and their mechanical development may proceed differently in weight-bearing and non-weight-bearing tendons. Further analysis of how this loading affects developing tendons in vivo may inform future engineering approaches aiming to apply such mechanical cues to regulate engineered tendon formation in vitro. (C) 2019 Elsevier Ltd. All rights reserved.