A novel bioactive nerve conduit for the repair of peripheral nerve injury.
A novel bioactive nerve conduit for the repair of peripheral nerve injury.
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一种用于修复周围神经损伤的新型生物活性神经导管
DOI：
10.4103/1673-5374.175062
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发表时间：
2016-01
影响因子：
6.1
通讯作者：
Li SP
中科院分区：
文献类型：
--
作者：
Li BB;Yin YX;Yan QJ;Wang XY;Li SP
神经导管的使用为周围神经再生提供了调节细胞因子、生长因子和神经营养因子的机会，避免了自体移植物的缺陷。我们构建了以聚乳酸-co-[（乙醇酸）-alt-（l -赖氨酸）]}和β-磷酸三钙修饰的聚d -l -乳酸（PDLLA）神经导管。将该生物活性pdlla神经导管与单纯pdlla神经导管在大鼠坐骨神经损伤后10 mm神经再生中的效果进行了比较。采用苏木精-伊红染色和亚甲基蓝染色，观察损伤后35 d再生早期的神经形态。与PDLLA导管相比，PDLLA生物活性神经导管的神经纤维更粗，尺寸更规则。PDLLA生物活性组比目鱼肌肌纤维直径大于单用PDLLA组。基于聚乳酸的生物活性神经导管是一种很有前途的坐骨神经损伤修复策略。
The use of a nerve conduit provides an opportunity to regulate cytokines, growth factors and neurotrophins in peripheral nerve regeneration and avoid autograft defects. We constructed a poly-D-L-lactide (PDLLA)-based nerve conduit that was modified using poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]} and β-tricalcium phosphate. The effectiveness of this bioactive PDLLA-based nerve conduit was compared to that of PDLLA-only conduit in the nerve regeneration following a 10-mm sciatic nerve injury in rats. We observed the nerve morphology in the early period of regeneration, 35 days post injury, using hematoxylin-eosin and methylene blue staining. Compared with the PDLLA conduit, the nerve fibers in the PDLLA-based bioactive nerve conduit were thicker and more regular in size. Muscle fibers in the soleus muscle had greater diameters in the PDLLA bioactive group than in the PDLLA only group. The PDLLA-based bioactive nerve conduit is a promising strategy for repair after sciatic nerve injury.