MODULATION OF MACROPHAGE INTERACTION WITH TRYPANOSOMA-CRUZI BY PHOSPHOLIPASE-A2-SENSITIVE COMPONENTS OF THE PARASITE MEMBRANE
MODULATION OF MACROPHAGE INTERACTION WITH TRYPANOSOMA-CRUZI BY PHOSPHOLIPASE-A2-SENSITIVE COMPONENTS OF THE PARASITE MEMBRANE
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DOI：
10.1016/0006-291x(84)90766-6
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
KIERSZENBAUM, F
中科院分区：
文献类型：
--
作者：
CONNELLY, MC;KIERSZENBAUM, F
磷脂酶A2 （PLA2）的存在显著增加了克氏锥虫与巨噬细胞之间的关联。这种效应反映了寄生虫膜的改变，因为它只在寄生虫而不是巨噬细胞用PLA2预处理时才会复制。特定底物磷脂酰胆碱阻断PLA2活性的能力表明，PLA2活性是显著增强的原因。PLA2抑制剂quinacrine、4-溴苯酰溴或芬特明的存在可显著抑制寄生虫与巨噬细胞的关联。Quinacrine也抑制寄生虫与非吞噬性宿主细胞的关联。这些结果表明内源性PLA2在克氏锥虫感染细胞的初始阶段起作用。
The presence of phospholipase A2 (PLA2) significantly increased the association between T. cruzi and macrophages [from mice]. This effect reflected alterations to the parasite membrane since it was reproduced only when the parasite but not the macrophage was pretreated with PLA2. That PLA2 activity was responsible for the noted enhancement was indicated by the ability of the specific substrate phosphatidylcholine to block it. The presence of the PLA2 inhibitors quinacrine, 4-bromophenacyl bromide or phentermine markedly inhibited parasite-macrophage association. Quinacrine also inhibited association of the parasite with a non-phagocytic host cell. These results suggested a role for endogenous PLA2 in the initial stages of cell infection by T. cruzi.