Embryonary Mouse Cardiac Fibroblast Isolation
Embryonary Mouse Cardiac Fibroblast Isolation
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DOI：
10.1007/978-1-4939-7714-7_7
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Ramirez, Israel
中科院分区：
文献类型：
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作者：
Garate-Carrillo, Alejandra;Ramirez, Israel
小鼠心脏成纤维细胞已被广泛用作研究心脏病理基础生物学过程和机制以及鉴定潜在治疗靶点的体外模型。心脏FB相对容易在培养皿中培养，并且可以使用分子和药理学工具进行操作。由于FB出生后迅速降低细胞周期分裂和增殖率，因此在几次传代后不久，它们在细胞培养物中容易发生表型变化和衰老。因此，来自胚胎的分化成纤维细胞的原代培养物是更期望的。下面我们将描述一种方法，该方法在原代培养物中提供E16 CD-1小鼠胚胎心脏成纤维细胞的良好细胞产量和活力。
Mouse cardiac fibroblasts have been widely used as an in vitro model for studying fundamental biological processes and mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Cardiac FBs are relatively easy to culture in a dish and can be manipulated using molecular and pharmacological tools. Because FBs rapidly decrease cell cycle division and proliferative rate after birth, they are prone to phenotypic changes and senescence in cell culture soon after a few passages. Therefore, primary cultures of differentiated fibroblasts from embryos are more desirable. Below we will describe a method that provides good cell yield and viability of E16 CD-1 mouse embryonic cardiac fibroblasts in primary cultures.