Immunoelectron microscopy of Alzheimer and Pick brain tissue labelled with the monoclonal antibody Alz-50.
Immunoelectron microscopy of Alzheimer and Pick brain tissue labelled with the monoclonal antibody Alz-50.
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用单克隆抗体 Alz-50 标记的阿尔茨海默氏症和 Pick 脑组织的免疫电子显微镜。
DOI：
10.1111/j.1365-2990.1989.tb01224.x
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发表时间：
1989
影响因子：
5
通讯作者：
Wiley,CA
中科院分区：
文献类型：
--
作者：
Love,S;Burrola,P;Terry,RD;Wiley,CA
先前的研究表明，阿尔茨海默病和皮克大脑含有异常升高的68 Kd蛋白质，该蛋白质由单克隆抗体Alz-50检测到。我们已经使用免疫过氧化物酶和免疫学技术本地化的Alz-50反应表位在部分阿尔茨海默氏症和挑选大脑的超微结构水平。可检测到的免疫反应性仅限于阿尔茨海默病神经原纤维缠结的成对螺旋丝和皮克体的成对螺旋丝和直丝。在阿尔茨海默病组织中，该抗体还标记了分散的神经元配对螺旋丝，这些螺旋丝没有聚集成神经元缠结。无定形成分的皮克机构和其他成分的阿尔茨海默病和皮克脑组织没有免疫染色。
Previous studies have shown that Alzheimer and Pick brains contain abnormally elevated amounts of a 68Kd protein detected by the monoclonal antibody Alz–50. We have used immunoperoxidase and immunological techniques to localize Alz–50–reactive epitopes in sections from Alzheimer and Pick brains at the ultrastructural level. Detectable immunoreactivity was restricted to the paired helical filaments of Alzheimer neurofibrillary tangles and to the paired helical and straight filaments of Pick bodies. In Alzheimer tissue, the antibody also labelled scattered neuronal paired helical filaments that were not aggregated into neurofibrillary tangles. Amorphous components of Pick bodies and other constituents in the Alzheimer and Pick brain tissue were not immunostained.