Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy.
Near-atomic resolution reconstructions of icosahedral viruses from electron cryo-microscopy.
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DOI：
10.1016/j.sbi.2011.01.008
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发表时间：
2011-04
影响因子：
6.8
通讯作者：
Harrison SC
中科院分区：
文献类型：
--
作者：
Grigorieff N;Harrison SC
在2008年初至2010年底期间发表的电子冷冻显微镜测定的九种不同的二十面体病毒的近原子分辨率结构，实现了15年前的预测，即单粒子冷冻电镜技术可以以3 - 4 Å分辨率显示分子细节。这篇综述总结了在仪器和计算方面的技术发展，这些技术发展导致了新的结构，这些结构促进了我们对病毒组装和细胞进入的理解。
Nine different near-atomic resolution structures of icosahedral viruses, determined by electron cryo-microscopy and published between early 2008 and late 2010, fulfill predictions made 15 years ago that single-particle cryo-EM techniques could visualize molecular detail at 3 – 4 Å resolution. This review summarizes technical developments, both in instrumentation and in computation, that have led to the new structures, which advance our understanding of virus assembly and cell entry.