Location of the Na+ cation in negative ions of DNA evidenced by using MS2 experiments in ion trap mass spectrometry
Location of the Na+ cation in negative ions of DNA evidenced by using MS2 experiments in ion trap mass spectrometry
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DOI：
10.1016/s1387-3806(99)00049-4
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发表时间：
1999-08-23
影响因子：
1.8
通讯作者：
Tabet, JC
中科院分区：
文献类型：
--
作者：
Favre, A;Gonnet, F;Tabet, JC
碰撞诱导解离（CID）光谱的去质子化的分子制备的各种单阳离子homoblitides和heteroblitides，使用外部nanoelectropray源与离子阱质谱仪，进行了说明。在这样的条件下，在负离子模式下实现的灵敏度足以从双电荷或三电荷负离子获得去质子化寡核苷酸的有用CID质谱。从子离子的分析结果表明，负电荷位于5 '-或3'-末端的网站，而盐基团是在一个中心位置。在CID过程中，阳离子似乎保持静止。此外，有证据表明，中心阳离子位点既不受寡核苷酸大小的影响，也不受5 ′-或3 ′-末端碱基性质的影响，这表明双电荷或三电荷准分子阴离子具有特别的稳定性。(Int J Mass Spectrom 190/191（1999）303-312）（C）1999 Elsevier Science B.V.
Collision-induced dissociation (CID) spectra of deprotonated molecules prepared from a variety of monocationized homonucleotides and heteronucleotides, using an external nanoelectrospray source with an ion trap mass spectrometer, are described. Under such conditions, the achieved sensitivity in the negative ion mode is sufficient to obtain useful CID mass spectra of deprotonated oligonucleotides from doubly or triply charged negative ions. Results from the analysis of the daughter ions show that the negative charges are located on 5'- or 3'-terminal sites whereas the salt group is at a central position. The cation appears to remain stationary during the CID process. Moreover, evidence exists that the central cation site is neither influenced by the size of the oligonucleotide nor by the nature of the 5'- or 3'-terminus base, which indicates particular stability for the doubly or triply charged quasimolecular anion. (Int J Mass Spectrom 190/191 (1999) 303-312) (C) 1999 Elsevier Science B.V.