Collagen-synthesizing cells in initial and advanced atherosclerotic lesions of human aorta
Collagen-synthesizing cells in initial and advanced atherosclerotic lesions of human aorta
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DOI：
10.1016/s0021-9150(96)06056-x
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发表时间：
1997-04-01
期刊：
影响因子：
5.3
通讯作者：
Orekhov, AN
中科院分区：
文献类型：
--
作者：
Andreeva, ER;Pugach, IM;Orekhov, AN
细胞外基质积聚、纤维化：被认为是动脉粥样硬化的主要表现之一。I型胶原是人动脉粥样硬化斑块中的主要基质成分。在这项工作中，我们已经证明了表达I型前胶原的细胞（PCl细胞），并研究了它们在大体正常的人主动脉和动脉粥样硬化病变中的定位：初始病变、脂肪条纹、纤维脂质病变（纤维脂质斑块、纤维粥样硬化）、纤维化病变（纤维斑块）。使用抗人I型前胶原的SPI.D8单克隆抗体进行免疫细胞化学显示PC 1细胞。我们未能在大体正常的主动脉和动脉粥样硬化病变下的介质中检测到PCl细胞。阳性细胞主要分布于初始病变区、脂肪条纹区、纤维脂质区和纤维斑块区。在脂肪条纹中发现最多的PCl细胞。这些细胞主要位于管腔前富含蛋白多糖的内膜亚层。大体正常区域的内膜细胞形成一个共同的细胞网络，通过突起相互连接。在动脉粥样硬化病变中发现细胞网络部分解体，这导致孤立细胞的出现。晚期病变中位于内膜细胞网络外部的分离的PCl细胞比例高于早期动脉粥样硬化病变区域。在初始病变中，大多数PC 1细胞被鉴定为平滑肌细胞，使用平滑肌α-肌动蛋白的抗体。在脂肪条纹中，表达PCl的平滑肌细胞数量较少。在纤维脂质和纤维斑块中发现用抗α-肌动蛋白和抗PCl抗体双染色的细胞少得多。这些双染色细胞的比例在细胞网络中涉及的PCl细胞总数中高于网络外的PCl细胞。研究结果表明，最活跃的从头合成的间质胶原湖的地方在动脉粥样硬化病变的区域，其特点是脂质沉积，这可能会导致动脉粥样硬化病变的进一步发展。(C)1997 Elsevier Science爱尔兰有限公司
Accumulation of extracellular matrix, fibrosis: is regarded to be one of the major manifestations of atherosclerosis. Collagen type I is the predominant matrix component in human atherosclerotic plaques. In this work we have demonstrated procollagen type I expressing cells (PCl-cells) and studied their localization in grossly normal human aorta and atherosclerotic lesions: initial lesions, fatty streaks, fibrolipid lesions (fibrolipid plaque, fibroatheroma), fibrotic lesions (fibrous plaque). PCl-cells were revealed immunocytochemically using SPI.D8 monoclonal antibody against human procollagen type I. We failed to detect PCl-cells in the areas of grossly normal aorta and media underlying atherosclerotic lesions. Positively stained cells were shown in the areas of initial lesions, fatty streaks, fibrolipid and fibrous plaques. The largest amount of PCl-cells was revealed in fatty streaks. These cells were predominantly localized in the preluminal proteoglycan-rich intimal sublayer. Intimal cells in grossly normal regions formed a common cellular network contacting each other with their processes. The cellular network is found to be partly disintegrated in atherosclerotic lesions, which leads to the appearance of isolated cells. The share of isolated PCl-cells localized outside the intimal cellular network was higher in advanced lesions than in the areas of early atherosclerotic lesions. In initial lesions most of PCl-cells were identified as smooth muscle cells using antibodies to smooth muscle cc-actin. Tn fatty streaks PCl-expressing smooth muscle cells were fewer in number. Much fewer cells double-stained with anti-alpha-actin and anti-PCl antibodies were found in fibrolipid and fibrous plaques. The proportion of these double stained cells was higher among total number of PCl-cells involved in the cellular network versus PCl-cells outside the network. The results of the study demonstrated that the most active de novo synthesis of interstitial collagen lakes place in the regions of atherosclerotic lesions characterized by lipid deposition, which may lead to the further progression of atherosclerotic lesions. (C) 1997 Elsevier Science Ireland Ltd.