Aggregate formation of zein and its structural inversion in aqueous ethanol
Aggregate formation of zein and its structural inversion in aqueous ethanol
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DOI：
10.1016/j.jcs.2007.08.004
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发表时间：
2008-01-01
影响因子：
3.8
通讯作者：
Xu, J.
中科院分区：
文献类型：
--
作者：
Kim, S.;Xu, J.
玉米蛋白是玉米的一种脯氨酸。通常，70-90%的水乙醇用于溶解玉米蛋白。用动态光散射仪监测乙醇水溶液中玉米蛋白分子的水动力半径，发现玉米蛋白在溶剂中存在聚集现象，其聚集程度与溶剂混合物的组成有关。当溶剂中乙醇含量从70%增加到90%时，玉米蛋白分子聚集数从1万个减少到最小。当溶剂混合物中乙醇含量增加到92%时，聚合数突然增加到1万以上。由于玉米蛋白具有两亲性，这种行为被解释为在其溶液中形成胶束状结构，其中约90%的乙醇显示最小的聚集数被视为结构反转点。这一观点得到了一个简单实验的支持，该实验表明玉米蛋白分子与亲水或疏水粒子有选择性的相互作用。Elsevier Ltd.出版。
Zein is a prolamine of maize. Conventionally, 70-90% aqueous ethanol is used to dissolve zein. When the hydrodynamic radii of zein molecules in aqueous ethanol were monitored with a dynamic light scattering instrument, it was found that zein aggregates in the solvent and the degree of aggregation depends on the composition of the solvent mixture. As the ethanol content of solvent increased from 70% to 90%, the aggregation number of zein molecules decreased from 10,000 until it reached a minimum. The aggregation number then increased abruptly to greater than 10,000 as the ethanol content of the solvent mixture increased to 92%. Since zein has amphiphilic characteristics, this behavior was interpreted as the formation of a micelle-like structure in its solution whereby ca. 90% ethanol that showed minimum aggregation number is regarded as a structural inversion point. This point of view was supported by a simple experiment that showed selective interaction of zein molecules with hydrophilic or hydrophobic particles. Published by Elsevier Ltd.