Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology
Isolation and characterization of 18 microsatellites for Aconitum vilmorinianum Kom. (Ranunculaceae) using next-generation sequencing technology
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DOI：
10.1007/s12686-015-0432-8
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发表时间：
2015-06-01
影响因子：
1.1
通讯作者：
Meng, Jing
中科院分区：
文献类型：
--
作者：
He, Jun;Zhang, Zhi-Rong;Meng, Jing
乌头是一种非常重要的中药植物，是云南白药作为镇痛消炎剂的主要原料之一。近年来，它受到过度开发和人类干扰的威胁。该物种的遗传背景对其有效保护和合理利用至关重要。因此，利用新一代测序技术为 A. vilmorinianum 开发了总共 18 个新型微卫星标记。每个位点的等位基因数量范围为 1 至 5，平均值为 2.560，观察到的和预期的杂合度范围分别为 0 至 1.000 和 0 至 0.733。这些多态性微卫星基因座对于这种重要药用植物的遗传学研究特别有用。
Aconitum vilmorinianum is a very important Chinese traditional medicinal plant, which is one of the main raw materials in Yunnan Baiyao as an analgesic and anti-inflammatory agent. Recently, it has been threatened by overexploitation and human disturbances. Genetic background of this species is essential for the efficient conservation and rational utilization. Therefore, a total of 18 novel microsatellite markers were developed for A. vilmorinianum using next-generation sequencing technology. The number of alleles per locus ranged from 1 to 5, with a mean of 2.560, and the observed and expected heterozygosities ranged from 0 to 1.000 and from 0 to 0.733, respectively. These polymorphic microsatellite loci will be especially useful for genetics studies of this important medicinal plant.