Interaction between Potassium Phosphate Buffer Solution and Modeling Cell Membrane Investigated by Sum Frequency Generation Vibrational Spectroscopy
Interaction between Potassium Phosphate Buffer Solution and Modeling Cell Membrane Investigated by Sum Frequency Generation Vibrational Spectroscopy
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通过和频产生振动光谱研究磷酸钾缓冲溶液与模拟细胞膜之间的相互作用
DOI：
10.1063/1674-0068/28/cjcp1504070
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发表时间：
2015-09
影响因子：
1
通讯作者：
Shuji Ye
中科院分区：
文献类型：
--
作者：
Weilai Yang;Kangzhen Tian;Shuji Ye
磷酸二氢钾缓冲溶液在生物实验中得到了广泛的应用，它代表了离子与生物分子相互作用的重要过程，但在分子水平上对磷酸二氢钾对细胞膜等生物分子的影响还知之甚少。在这项工作中，我们应用和频产生振动光谱技术，实时研究了磷酸二氢钾缓冲溶液与负1，2-dimyristoyl-d54-sn-glycero-3-[phospho-rac-(1-glycerol)](d54-DMPG)脂双分子膜的相互作用。首次利用CD2、CD3、头磷脂和羧基的光谱特征监测了PBS诱导的d54-DMPG脂双层分子结构的动态变化。结果发现，K+能迅速与细胞膜结合，引起CD2、CD3、脂头磷酸盐和羧基的信号变化。钾PbS与脂质双分子层的相互作用很可能是通过形成...
Potassium phosphate buffer solution has been widely used in the biological experiments, which represents an important process of the interaction between ions and biomolecules, yet the influences of potassium phosphate on biomolecules such as the cell membrane are still poorly understood at the molecular level. In this work, we have applied sum frequency generation vibrational spectroscopy and carried out a detailed study on the interaction between potassium phosphate buffer solution (PBS) and negative 1,2-dimyristoyl-d54-sn-glycero-3-[phospho-rac-(1-glycerol)] (d54-DMPG) lipid bilayer in real time. The PBS-induced dynamic change in the molecular structure of d54-DMPG lipid bilayer was monitored using the spectral features of CD2, CD3, lipid head phosphate, and carbonyl groups for the first time. It is found that K+ can bind to the cell membrane and cause the signal change of CD2, CD3, lipid head phosphate, and carbonyl groups quickly. Potassium PBS interacts with lipid bilayers most likely by formation of...
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影响因子：
3
作者：
Ye, Shuji;Nguyen, Khoi Tan;Le Clair, Stephanie V.;Chen, Zhan
通讯作者：
Chen, Zhan
影响因子：
8.9
作者：
E. Leontidis;M. Christoforou;C. Georgiou;Thomas Delclos
通讯作者：
E. Leontidis;M. Christoforou;C. Georgiou;Thomas Delclos
影响因子：
4.4
作者：
Gurtovenko, AA
通讯作者：
Gurtovenko, AA
影响因子：
2.4
作者：
Woongmo Sung;Doseok Kim;Y. Shen
通讯作者：
Woongmo Sung;Doseok Kim;Y. Shen
影响因子：
3.7
作者：
Feng Wei;Hongchun Li;Shuji Ye
通讯作者：
Feng Wei;Hongchun Li;Shuji Ye