Induction of c-myc expression in human B lymphocytes by B-cell growth factor and anti-immunoglobulin.
Induction of c-myc expression in human B lymphocytes by B-cell growth factor and anti-immunoglobulin.
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B 细胞生长因子和抗免疫球蛋白诱导人 B 淋巴细胞中的 c-myc 表达。
DOI：
10.1073/pnas.83.5.1458
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发表时间：
1986
影响因子：
11.1
通讯作者：
Summers,WC
中科院分区：
文献类型：
--
作者：
Lacy,J;Sarkar,SN;Summers,WC
用35 S标记的DNA探针原位杂交法检测了纯化的人B淋巴细胞对促有丝分裂刺激的c-myc转录表达。与静息B细胞相比，在加入抗mu、福尔马林化金黄色葡萄球菌科万菌株I或B细胞生长因子后2小时内，c-myc表达水平增加了10- 20倍。在促有丝分裂刺激72-96小时后，c-myc表达仍然升高5倍，但单个细胞之间的表达比早期时间点变得更加异质。为了确定c-myc在人B淋巴细胞中的表达在细胞周期内是否是时相特异性的，通过DNA含量将促分裂原刺激的细胞分选成细胞周期的G 0/G1、S和G2/M期的细胞群。检查c-myc在特定阶段的细胞中的表达显示，c-myc的表达在细胞周期的所有阶段都升高，但它似乎是最大的表达在S期。这些研究表明，正常人B淋巴细胞中的c-myc表达是细胞周期依赖性的，并且在细胞周期的所有阶段都保持升高。
Purified human B lymphocytes were examined for transcriptional expression of c-myc in response to mitogenic stimulation by the method of in situ hybridization using 35S-labeled DNA probes. The level of c-myc expression increased 10- to 20-fold within 2 hr after the addition of anti-mu, formalinized Staphylococcus aureus Cowan strain I, or B-cell growth factor, as compared to resting B cells. After 72-96 hr of mitogenic stimulation, c-myc expression remained elevated 5-fold, but expression among individual cells had become more heterogeneous than at early time points. To determine whether c-myc expression in human B lymphocytes is phase specific within the cell cycle, mitogen-stimulated cells were sorted by DNA content into populations of cells in G0/G1, S, and G2/M phases of the cell cycle. Examination of c-myc expression in phase-specific cells revealed that c-myc expression was elevated in all phases of the cell cycle, but it appeared to be maximally expressed in S phase. These studies suggest that c-myc expression in normal human B lymphocytes is cell-cycle dependent and remains elevated in all phases of the cycling cell.