Separation of long DNA molecules in a microfabricated entropic trap array
Separation of long DNA molecules in a microfabricated entropic trap array
复制标题
DOI：
10.1126/science.288.5468.1026
复制
发表时间：
2000-05-12
期刊：
影响因子：
56.9
通讯作者：
Craighead, HG
中科院分区：
文献类型：
--
作者：
Han, J;Craighead, HG
设计并制作了一种用于分离长DNA分子的纳米流体通道装置，该装置由多个熵陷阱组成。该通道包括狭窄的狭窄和较宽的区域，这些区域在狭窄开始时导致DNA的大小依赖于捕获。这一过程产生了电泳迁移率的差异，从而能够在不使用凝胶基质或脉冲电场的情况下实现高效分离。长DNA分子(5000到类似于16万个碱基对)的样品在15毫米长的通道中被有效地分离成条带。演示了多通道器件的并联运行。该装置的高效、紧凑和易于制造表明了更实用的集成DNA分析系统的可能性。
A nanofluidic channel device, consisting of many entropic traps, was designed and fabricated for the separation of Long DNA molecules. The channel comprises narrow constrictions and wider regions that cause size-dependent trapping of DNA at the onset of a constriction. This process creates electrophoretic mobility differences, thus enabling efficient separation without the use of a gel matrix or pulsed electric fields. Samples of Long DNA molecules (5000 to similar to 160,000 base pairs) were efficiently separated into bands in 15-millimeter-long channels. Multiple-channel devices operating in parallel were demonstrated. The efficiency, compactness, and ease of fabrication of the device suggest the possibility of more practical integrated DNA analysis systems.