Differential expression of VIP/PACAP receptor genes in breast, intestinal, and pancreatic cell lines.
Differential expression of VIP/PACAP receptor genes in breast, intestinal, and pancreatic cell lines.
复制标题
VIP/PACAP 受体基因在乳腺、肠和胰腺细胞系中的差异表达。
DOI：
10.1016/0304-3835(95)03768-r
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发表时间：
1995
期刊：
影响因子：
9.7
通讯作者：
Bravo,DT
中科院分区：
文献类型：
--
作者：
Waschek,JA;Richards,ML;Bravo,DT
血管活性肠肽（VIP）和垂体腺苷酸环化酶激活肽（PACAP）是结构相关的神经肽，除了作为神经递质的经典作用外，还作为营养因子发挥作用。结合和分子克隆研究表明，它们的作用由至少三种不同基因编码的受体介导。VIP结合已被证明在许多肿瘤类型，放射性标记的VIP最近已被用作一种新的方法来定位在人类肠道肿瘤及其转移部位。为了确定受体亚型和基因表达水平，我们通过北方印迹分析筛选了乳腺、肠和胰腺细胞系。乳腺细胞系表达的VIP/PACAP 1受体mRNA水平与肠道细胞系相当，这与研究结果一致，研究显示这些肿瘤及其衍生细胞系中VIP结合特别高。胰腺细胞系表达几种受体类型的mRNA。这扩展了VIP和PACAP在肿瘤定位中的潜在效用，并且由于VIP和PACAP可以通过自分泌或其他机制调节某些肿瘤的生长速率，因此这些细胞系上的受体亚型的鉴定为研究这些个体受体在生长和其他过程中的活性奠定了基础。
Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) are structurally-related neuropeptides that function as trophic factors in addition to their more classical roles as neurotransmitters. Binding and molecular cloning studies have shown that their actions are mediated by receptors encoded by at least three different genes. VIP binding has been demonstrated on many tumor types, and radiolabeled VIP has recently been used as a novel method to localize intestinal tumors in humans and their sites of metastasis. To determine the receptor subtype and level of gene expression, we screened breast, intestinal, and pancreatic, cell lines by Northern blot analysis. Breast lines expressed VIP/PACAP1receptor mRNA levels comparable to intestinal lines, in agreement with the studies showing particularly high VIP binding in these tumors and their derived cell lines. Pancreatic cell lines expressed mRNA for several receptor types. This extends the potential utility of VIP and PACAP in the localization of tumors, and because VIP and PACAP may regulate the growth rate of some tumors by autocrine or other mechanisms, the identification of receptor subtypes on these lines sets the stage for studies in which the activity of these individual receptors in growth and other processes can be investigated.