Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP
Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP
复制标题
NFκB、MAPKs 和 IRF3 通路下游的 Akt 参与黄芪多糖 RAP 诱导的巨噬细胞活化
DOI：
10.1016/j.jff.2017.10.004
复制
发表时间：
2017-12-01
影响因子：
5.6
通讯作者：
Han, Quan-Bin
中科院分区：
文献类型：
--
作者：
Li, Zhi-Peng;Li, Li-Feng;Han, Quan-Bin
黄芪多糖被认为是黄芪增强免疫功能的主要活性成分，是常用的药用食品辅料。参与免疫增强作用的信号通路尚不清楚。本研究旨在探讨Akt通路与TLR4受体下游激酶在黄芪多糖RAP诱导的巨噬细胞中的作用及调控关系。结果表明，RAP诱导RAW264.7细胞中Akt、NF kappa B、IRF3和MAPKs的磷酸化，其单独抑制剂不仅抑制其炎症因子的产生和基因表达，还抑制Akt的磷酸化，而Akt抑制剂对MAPKs、NF kappa B和IRF3的磷酸化无影响。综上所述，这些结果表明Akt和IRF3在RAP诱导的巨噬细胞中发挥激活作用，Akt在MyD88依赖和独立通路下游发挥作用。该研究将为黄芪多糖诱导的信号转导提供更深入的认识。(C) 2017 Elsevier Ltd.版权所有。
Polysaccharides are considered the main active ingredient for the immune-enhancing effect of Radix Astragali, which is popularly used as medicinal food supplementary. The signal pathways involved in immune-enhancing effect remain unclear. This study is to investigate the role and regulation relation of Akt pathway with downstream kinases of TLR4 receptor in macrophages induced by Astragalus polysaccharide, RAP. The results showed that RAP induced phosphorylation of Akt, NF kappa B, IRF3 and MAPKs in RAW264.7 cells, and their individual inhibitors not only inhibited their inflammatory factors production and gene expression, but also suppressed the Akt phosphorylation, while Akt inhibitor exerted no effect on MAPKs, NF kappa B, and IRF3 phosphorylation. Taken together, these results suggested that Akt and IRF3 exerted activation role in RAP induced macrophage, and Akt exerted effect downstream of MyD88 dependent and independent pathway. This study will provide a deeper insight into the signal transduction induced by Astragalus polysaccharides. (C) 2017 Elsevier Ltd. All rights reserved.