Low-level expression of purine bases in BALB/3T3 cells monitored by ultrasensitive graphene-based glass carbon electrode.
Low-level expression of purine bases in BALB/3T3 cells monitored by ultrasensitive graphene-based glass carbon electrode.
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DOI：
10.1016/j.ab.2014.08.034
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发表时间：
2014-12
影响因子：
2.9
通讯作者：
Guang-gang Gao;Guo-Biao Xu;Jin-lian Li;Jiwen Cui;Dongmei Wu;H. Qiu
中科院分区：
文献类型：
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作者：
Guang-gang Gao;Guo-Biao Xu;Jin-lian Li;Jiwen Cui;Dongmei Wu;H. Qiu
采用超灵敏化学还原氧化石墨烯和离子液体修饰玻璃碳电极（RGI-GCE），观察了腺嘌呤和次黄嘌呤在人乳腺癌（MCF-7）和小鼠胚胎成纤维细胞（BALB/3T3）中分离的电化学信号。首次可以电化学监测非癌性BALB/3T3细胞中嘌呤碱基的低水平表达。通过不同嘌呤碱基的电化学信号变化，研究了致癌物质污染的BALB/3T3细胞中嘌呤碱基的代谢，为探索非癌细胞中低水平嘌呤的机制开辟了新的电化学途径。
Using an ultrasensitive chemically reduced graphene oxide and ionic liquid modified glass carbon electrode (RGI–GCE), separated electrochemical signals of adenine and hypoxanthine in both human breast cancer (MCF-7) and mouse embryonic fibroblast (BALB/3T3) cells were observed. For the first time, low-level expression of purine bases in noncancerous BALB/3T3 cells can be electrochemically monitored. The metabolism of purine bases in carcinogen agent-contaminated BALB/3T3 cells was also investigated through the change of electrochemical signals ascribed to different purine bases, which opens a new electrochemical approach to the exploration of a low-level purine mechanism in noncancerous cells.