Successful hematopoietic reconstitution with transplantation of erythrocyte-depleted allogeneic human umbilical cord blood cells in a child with leukemia.
Successful hematopoietic reconstitution with transplantation of erythrocyte-depleted allogeneic human umbilical cord blood cells in a child with leukemia.
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通过移植红细胞耗尽的同种异体人脐带血细胞，对患有白血病的儿童成功进行造血重建。
DOI：
10.1073/pnas.91.10.4485
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发表时间：
1994
影响因子：
11.1
通讯作者：
Good,RA
中科院分区：
文献类型：
--
作者：
Pahwa,RN;Fleischer,A;Than,S;Good,RA
脐带血是造血干细胞的有效来源，已被证明在各种疾病的异基因移植后成功地重建造血。脐带血的一个主要缺点是ABO血型不相容的输血反应的风险，以及如果脐带血在冷冻保存前或解冻后被操作以去除红细胞，则干细胞库急剧减少。本文介绍了一种采用3%明胶诱导红细胞沉降法从脐带血中选择性去除红细胞的方法。红细胞耗尽的部分被证明是丰富的祖细胞和细胞分泌造血细胞因子白细胞介素3，粒细胞/巨噬细胞集落刺激因子，白细胞介素6，细胞因子的主要来源是从脐带T细胞。我们用这种制备技术从一名剖宫产婴儿的脐带血中分离出红细胞，该婴儿有一个8岁的白血病兄弟姐妹。脐带细胞的组织相容性测试显示与患者完全匹配。在清髓性化疗后，对患者进行了冻存和解冻细胞的脐带细胞移植，其中包括4 × 107个有核细胞/kg。患者的快速血液学恢复和迄今为止9个月的无病期表明，3%明胶分离红细胞是一种简单的方法，可以成功地用于移植脐带细胞治疗恶性/非恶性疾病。
Cord blood, a potent source of hematopoietic stem cells, has been shown to successfully reconstitute hematopoiesis following allogeneic transplantation in a variety of disorders. A major drawback of cord blood has been the risk of transfusion reactions in ABO blood group incompatibility and drastic reduction in the stem cell pool if the cord blood is manipulated to remove red cells prior to cryopreservation or after thawing. This report describes an erythrocyte depletion method employing 3% gelatin-induced erythrocyte sedimentation for the selective removal of red cells from cord blood. The red cell-depleted fraction was shown to be enriched in progenitor cells and in cells secreting hematopoietic cytokines interleukin 3, granulocyte/macrophage colony-stimulating factor, and interleukin 6; a major source for cytokines was from cord T cells. This preparative technique was employed to separate out red cells from cord blood of an infant delivered by cesarean section who had an 8-year-old sibling with leukemia. Histocompatibility testing of cord cells revealed complete matching with the patient. A cord cell transplant of cryopreserved and thawed cells consisting of 4 x 10(7) nucleated cells per kg was administered to the patient following myeloablative chemotherapy. The patient's quick hematologic recovery and 9-month disease-free period to date suggest that 3% gelatin separation of erythrocytes is a simple method that can be successfully used for transplanting cord cells for malignant/nonmalignant diseases.