Antihyperglycemic effect of esculetin modulated carbohydrate metabolic enzymes activities in streptozotocin induced diabetic rats
Antihyperglycemic effect of esculetin modulated carbohydrate metabolic enzymes activities in streptozotocin induced diabetic rats
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DOI：
10.1016/j.jff.2012.05.005
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发表时间：
2012-10-01
影响因子：
5.6
通讯作者：
Ashokkumar, Natarajan
中科院分区：
文献类型：
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作者：
Prabakaran, Dhamodaran;Ashokkumar, Natarajan
2型糖尿病(T2 DM)是一种世界性的公共卫生挑战。本研究旨在探讨七叶内酯对链脲佐菌素(STZ)诱导的糖尿病大鼠糖代谢关键酶的影响。先用链脲佐菌素(40 mg/kgbw)诱导大鼠糖尿病模型，然后给予不同浓度的秦皮乙素(10、20、40 mg/kgbw)治疗45d。糖尿病大鼠血糖和糖化血红蛋白(HbA(1c))水平升高，血浆胰岛素和血红蛋白(Hb)水平下降。同时测定食物摄入量、水摄入量和口服葡萄糖耐量试验。葡萄糖-6-磷酸酶、果糖-1，6-二磷酸酶等碳水化合物代谢酶活性显著升高，葡萄糖-6-磷酸脱氢酶、葡萄糖-6-磷酸脱氢酶活性显著降低。秦皮乙素能显著降低血糖、糖化血红蛋白(1c)，升高糖化血红蛋白和胰岛素水平。葡萄糖激酶、葡萄糖-6-磷酸脱氢酶等关键酶活性显著升高，葡萄糖-6-磷酸酶、果糖-1，6-二磷酸酶活性显著降低。此外，还观察了对糖尿病大鼠体重减轻的保护作用。在三种剂量中，秦皮乙素40 mg/kg bw对STZ诱导的糖尿病大鼠的降糖作用更为明显。(C)2012爱思唯尔有限公司。保留所有权利。
Type 2 diabetes mellitus (T2DM) being a worldwide challenge to public health. The aim of this study was to examine the effect of esculetin, a major compound present in the herbal plant of Matricaria chamomilla L., on the key enzymes of carbohydrate metabolism in streptozotocin (STZ) induced diabetic rats. At first, the rats were induced intraperitonial injection of STZ (40 mg/kg bw) to attain diabetes and were then treated with different concentrations of esculetin (10, 20 and 40 mg/kg bw) for 45 days. The diabetic rats had elevated levels of plasma glucose and glycated haemoglobin (HbA(1c)) and decreased plasma insulin and haemoglobin (Hb) levels. Food intake, water intake and oral glucose tolerance test were also measured. Activities of carbohydrate metabolic enzymes such as glucose-6-phosphatase, fructose 1,6-bisphosphatase increased and glucokinase, glucose-6-phosphate dehydrogenase decreased significantly. Oral administration of esculetin significantly decreased the levels of plasma glucose, HbA(1c) and increased the levels of Hb and insulin. The activities of the key enzymes such as glucokinase and glucose-6-phosphate dehydrogenase had significantly increased whereas, glucose-6-phosphatase and fructose-1,6-bisphosphatase had significantly decreased. Further, protection against body weight loss of diabetic rats was observed. Among the three doses, 40 mg/kg bw of esculetin exerted a more pronounced antihyperglycemic effect against STZ-induced diabetic rats. (c) 2012 Elsevier Ltd. All rights reserved.