Measurement of O2 consumption, CO2 production, and water vapor production in a closed system.
Measurement of O2 consumption, CO2 production, and water vapor production in a closed system.
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DOI：
10.1152/jappl.1987.62.5.2103
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发表时间：
1987-05
影响因子：
3.3
通讯作者：
D. Vleck
中科院分区：
文献类型：
--
作者：
D. Vleck
提出了用于计算恒容封闭系统呼吸计中 O2 消耗量、CO2 产生量和水蒸气产生量的方程。必要的测量仅包括呼吸计室中的初始温度、压力和气体体积，以及在测量开始和结束时采集的气体样本中的 O2 分数浓度。确定了因二氧化碳和水蒸气浓度变化而导致的潜在误差。忽略 CO2 影响可能会导致 O2 消耗量估计出现高达 6.4% 的误差，而水蒸气影响导致的误差可能超过 100%。提出了最小化潜在误差以及使用 O2 分析仪测量 CO2 和水蒸气浓度的技术，以便消除潜在误差。
Equations for the calculation of O2 consumption, CO2 production, and water vapor production in a constant-volume, closed-system respirometer are presented. Necessary measurements include only the initial temperature, pressure, and gas volume in the respirometer chamber, and the fractional concentration of O2 in gas samples taken at the beginning and end of the period of measurement. Potential errors resulting from changes in CO2 and water vapor concentrations are identified. Ignoring CO2 effects can produce up to a 6.4% error in estimates of O2 consumption, and errors due to water vapor effects can exceed 100%. Techniques are presented for minimizing potential errors and for measuring CO2 and water vapor concentrations with an O2 analyzer so that potential errors can be eliminated.