Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification
Amperometric biosensor for microRNA based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification
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DOI：
10.1007/s00604-017-2246-8
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发表时间：
2017-04
影响因子：
5.7
通讯作者：
Yan Huang;Shi Mo;Z. Gao;J. Chen;J. Lei;H. Luo;N. Li
中科院分区：
文献类型：
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作者：
Yan Huang;Shi Mo;Z. Gao;J. Chen;J. Lei;H. Luo;N. Li
MicroRNAs是内源性非编码rna，在生物过程中起着关键作用，可以被认为是疾病早期诊断和发病机制的分子标记。作者描述了一种基于使用四面体DNA纳米结构探针和鸟嘌呤纳米线扩增的高灵敏度的微rna电化学生物传感器。DNA四面体探针在金电极上自组装，提高了反应性、可及性和分子识别效率。结合四面体探针，鸟嘌呤纳米线放大了信号，提高了生物传感器的分析性能。该传感器的最佳工作电压通常为150 mV（相对于Ag/AgCl），在500 f至10 nM范围内对对数microRNA浓度具有线性响应，检测限为176 f。它具有很高的选择性，可以应用于实际样品。该方法在临床诊断和生物学研究中具有良好的应用前景。基于四面体DNA纳米结构的安培生物传感器耦合鸟嘌呤纳米线扩增用于分析microRNA-21的示意图。该策略具有很高的选择性，并且在检测乳腺癌患者的microRNA水平方面也表现良好。
MicroRNAs are endogenous noncoding RNAs that play critical roles in biological processes and can be considered as molecular markers for early diagnosis and pathogenesis of diseases. The authors describe a highly sensitive electrochemical biosensor for microRNA that is based on the use of tetrahedral DNA nanostructure probes and guanine nanowire amplification. The DNA tetrahedral probe is self-assembled on a gold electrode and enhances reactivity, accessibility, and molecular recognition efficiency. Combined with the tetrahedral probe, the guanine nanowire amplifies the signal and improves the analytical performance of the biosensor. Operated best at a voltage of typically 150 mV (vs. Ag/AgCl), the sensor has a linear response to the logarithmic microRNA concentration in the 500 f. to 10 nM range, with a 176 f. detection limit. It is highly selective and can be applied to real samples. It is concluded that this strategy has a good potential with respect to the determination of microRNA in clinical diagnosis and in biological research.Graphical abstractSchematic of a tetrahedral DNA nanostructure-based amperometric biosensor coupled to guanine nanowire amplification for analysis of microRNA-21. This strategy is highly selective and also performs well for the detection of microRNA levels of breast cancer patients.