Activity-dependent change in the protein level of brain-derived neurotrophic factor but no change in other neurotrophins in the visual cortex of young and adult ferrets
Activity-dependent change in the protein level of brain-derived neurotrophic factor but no change in other neurotrophins in the visual cortex of young and adult ferrets
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DOI：
10.1016/s0306-4522(02)00771-6
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Tsumoto, T
中科院分区：
文献类型：
--
作者：
Ichisaka, S;Katoh-Semba, R;Tsumoto, T
神经营养因子可能在视皮层发育的关键期起着调节视皮层活动性可塑性的作用。因此，预期皮质中神经营养因子的浓度随着发育和/或神经元活动的改变而变化。为了验证这一点，我们测量了蛋白质水平的神经生长因子，脑源性神经营养因子，神经营养因子-3和神经营养因子-4/5在视觉皮层的年轻（出生后38-46天，在高峰期的关键期）和成年雪貂与双位点酶免疫系统。测量也在躯体感觉皮层、海马和小脑中进行作为对照。随着发育，脑源性神经营养因子的水平没有显着变化，而其他神经营养因子的变化在视觉皮层。通过向双眼注射河豚毒素阻断视觉输入24 h，可显著降低视觉皮层中脑源性神经营养因子蛋白水平，但在年轻和成年雪貂的其他区域中不存在。另一方面，未观察到幼年和成年雪貂视觉皮层中其他神经营养因子的蛋白质水平显著降低。单眼注射河豚毒素在年轻的雪貂导致脑源性神经营养因子减少约一半，通过双眼注射。注射眼的对侧皮质的减少程度显著大于同侧皮质，反映了对侧眼在雪貂的皮质中占主导地位。GABA（A）受体激动剂阻断皮层神经元活动导致视觉皮层脑源性神经营养因子蛋白显著减少。这些结果表明，视皮层脑源性神经营养因子蛋白水平受皮层神经元活动的调节。(C)2003年IBRO。由Elsevier Science Ltd.出版，版权所有。
Neurotrophins are suggested to play a role in activity-dependent plasticity of visual cortex during the critical period of postnatal development. Thus, the concentration of neurotrophins in the cortex is expected to change with development and/or with alteration in neuronal activities. To test this, we measured protein levels of nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4/5 in visual cortex of young (postnatal day 38-46, at the peak of the critical period) and adult ferrets with two-site enzyme-immunoassay systems. Measurements were carried out also in somatosensory cortex, hippocampus and cerebellum as control. With development the level of brain-derived neurotrophic factor did not significantly change, while those of the other neurotrophins changed in the visual cortex. A blockade of visual inputs for 24 h by an injection of tetrodotoxin into both eyes significantly decreased brain-derived neurotrophic factor protein level in the visual cortex, but not in the other regions in both young and adult ferrets. On the other hand, no significant decrease was seen in the protein level of the other neurotrophins in the visual cortex of young and adult ferrets. A monocular injection of tetrodotoxin in young ferrets resulted in the reduction of brain-derived neurotrophic factor by approximately half that by binocular injection. The degree of the decrease in the contralateral cortex to the injected eye was significantly larger than that in the ipsilateral cortex, reflecting that the contralateral eye is dominantly represented in the cortex in ferrets. Blockade of cortical neuronal activities by a GABA(A) receptor agonist led to a remarkable reduction of brain-derived neurotrophic factor protein in the visual cortex. These results suggest that the level of brain-derived neurotrophic factor protein in visual cortex is regulated by activities of cortical neurons. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.