SECRETORY DYNAMICS OF ESTRADIOL (E2) AND PROGESTERONE (P4) DURING PERIODS OF RELATIVE PITUITARY LH QUIESCENCE IN THE MIDLUTEAL PHASE OF THE MENSTRUAL-CYCLE
SECRETORY DYNAMICS OF ESTRADIOL (E2) AND PROGESTERONE (P4) DURING PERIODS OF RELATIVE PITUITARY LH QUIESCENCE IN THE MIDLUTEAL PHASE OF THE MENSTRUAL-CYCLE
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DOI：
10.1111/j.1365-2265.1990.tb03745.x
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发表时间：
1990-01-01
影响因子：
3.2
通讯作者：
YEN, SSC
中科院分区：
文献类型：
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作者：
ROSSMANITH, WG;LAUGHLIN, GA;YEN, SSC
虽然脉冲式垂体促黄体生成素（LH）分泌和黄体释放类固醇激素之间的时间关系已被研究，雌二醇（E2）和孕酮（P4）的分泌曲线在没有任何明显的LH脉冲活动期间仍然未知。因此，在16名妇女的周期的黄体中期（周期中期LH峰后6-8天），每隔15分钟采集一次血样，持续24小时。在所有样本中测量LH，并通过聚类脉冲算法分析显著脉冲。在所有样本中，还评估了9项显示最低LH脉冲频率和大LH脉冲幅度的研究的E2和P4。这三种激素都是以脉动的方式释放的。发现E2和P4脉冲是同步的。而E2的释放频率（平均值±. SEM：8.9 ±。0.7脉冲/24小时）和P4（8.5 ±. 0.7脉冲/24小时）是可比的，LH脉冲频率（4.6 ±. 0.4脉冲/24 h）显著低于卵巢类固醇脉冲频率（P < 0.001）。最大（P < 0.01）交叉相关系数在28.1 ±-的正时滞处确定。7.7 LH/E2和31.7 ±. 5.8 LH/P4的变化在30 min内，表明E2或P4水平的变化往往发生在LH浓度变化后约30 min内。此外，类固醇脉冲和LH峰之间的伴随程度远高于偶然（P < 0.001）。E_2和P_4激素序列间的互相关系数在零时滞时达到最大值（P < 0.01），表明这些性激素是同时分泌的。在大幅度LH脉冲（> 5 IU/l）之前的E2或P4脉冲的脉冲幅度、脉冲持续时间和峰下面积明显大于（所有比较P < 0.05或更小）与之前LH脉冲无关的类固醇脉冲。因此，观察到两个类固醇脉冲群;一个与先前LH脉冲相关，并且具有所有脉冲属性（持续时间、幅度、峰下面积）的较大幅度，另一个与先前LH脉冲无关，并且具有较低幅度的脉冲特征。这一观察结果表明，卵巢类固醇激素的脉冲式释放是LH的间歇性调节影响的结果，并且脉冲式类固醇激素分泌在LH脉冲性的相对垂体静止期间具有较小的幅度。
Although the temporal relationship between pulsatile pituitary luteinizing hormone (LH) secretion and steroid hormone release from the corpus luteum has been investigated, the secretory profiles of oestradiol (E2) and progesterone (P4) during periods without any discernible LH pulsatile activity remain unknown. Consequently, blood was sampled at 15-min intervals for 24 h from 16 women during the midluteal phases (6-8 days after midcycle LH surge) of their cycles. LH was measured in all samples and analysed for significant pulses by the Cluster pulse algorithm. Nine studies showing the lowest LH pulse freqencies and large LH pulse amplitudes were also assessed for E2 and P4 in all samples. All three hormones were released in pulsatile fashions. Pulses of E2 and P4 were found to be syncrhonous. While the release frequencies for E2 (mean .+-. SEM: 8.9 .+-. 0.7 pulses/24 h) and P4 (8.5 .+-. 0.7 pulses/24 h) were comparable, the LH pulse frequency (4.6 .+-. 0.4 pulses/24 h) was found to be significantly (P < 0.001) lower than the ovarian steroid pulse frequencies. Maximum (P < 0.01) cross-correlation coefficients were determined at positive time lags of 28.1 .+-. 7.7 min for LH/E2 and 31.7 .+-. 5.8 min for LH/P4, indicating that changes in E2 or P4 levels tended to occur within approximately 30 min following LH concentration changes. Further, the degree of concomitance between a steroid pulse and an LH peak was much higher (P < 0.001) than by chance. Maximum (P < 0.01) cross-correlation coefficients between E2 and P4 hormonal data series were found at zero time lag, suggesting that these sex steroids were secreted simultaneously. The pulse amplitudes, pulse durations and areas under the peaks of those E2 or P4 pulses preceded by large (> 5 IU/l) amplitude LH pulse were significantly greater (P < 0.05 or less for all comparisons) than for steroid pulses not associated with preceding LH pulses. Thus, two populations of steroid pulses were observed; one associated with preceding LH pulses and having greater magnitude of all pulse attributes (duration, amplitude, area under the peaks), and another, not associated with preceding LH pulses and having pulse characteristics of lower magnitude. This observation suggests that the pulsatile release of ovarian steroids is a result of the episodic modulating influence of LH and that pulsatile steroid hormone secretion pertains with smaller magnitude during periods of relative pituitary quiescence of LH pulsatility.