Effectiveness of Increasing Temperature of Rearing Water and Non-feeding against Viral Endothelial Cell Necrosis of Eel
Effectiveness of Increasing Temperature of Rearing Water and Non-feeding against Viral Endothelial Cell Necrosis of Eel
复制标题
提高饲养水温和停饲对鳗鱼病毒性内皮细胞坏死的效果
DOI：
10.3147/jsfp.43.79
复制
发表时间：
2008
期刊：
影响因子：
0.6
通讯作者：
S. Ono
中科院分区：
文献类型：
--
作者：
Makoto Tanaka;Takayuki Satoh;Wenqian Ma;S. Ono
由腺病毒（JEAdV）引起的日本鳗鱼病毒性内皮细胞坏死（VECNE）一直是日本水产养殖业的严重问题。在本研究中，评估了提高水温和不喂食对抗 VECNE 的有效性。在20℃至31℃范围内，腹腔注射105.05TCID50/鱼的JEAdV的鱼的累积死亡率随着水温的升高而增加，但35℃时的死亡率与20℃时一样低。在不喂食的条件下饲养受感染的鱼进一步增强了35°C的处理效果。有效性取决于高温下的饲养时间；需要在 35°C 下放置 3 天以上才能降低死亡率。在 35°C 下经 JEAdV 初次攻击后存活下来的鱼，对 JEAdV 再次攻击表现出高度抵抗力。从这些结果来看，在 35°C 的非喂食条件下处理鱼对于控制 VECNE 是有用的。
Viral endothelial cell necrosis of eel (VECNE) of Japanese eel Anguilla japonica, caused by an adenovirus (JEAdV), has been a serious problem of aquaculture industry in Japan. In the present study, effectiveness of increasing water temperature and non-feeding against VECNE was evaluated. Cumulative mortalities of fish intraperitoneally injected with 105.05 TCID50/fish of JEAdV increased with elevating water temperature in the range between 20°C and 31°C, but mortality at 35°C was as low as that at 20°C. Rearing infected fish under the non-feeding condition further enhanced the effect of treatment at 35°C. The effectiveness was dependent on rearing periods at the high temperature; more than 3 days at 35°C were needed to reduce mortality. Fish, which survived the primary challenge with JEAdV at 35°C, showed high resistance to re-challenge with JEAdV. From these results, the treatment of fish under the non-feeding condition at 35°C is useful to control VECNE.