Tumor metastases and cell‐mediated immunity in a model system in DBA/2 mice. I. Tumor invasiveness in vitro and metastasis formation in vivo
Tumor metastases and cell‐mediated immunity in a model system in DBA/2 mice. I. Tumor invasiveness in vitro and metastasis formation in vivo
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DBA/2 小鼠模型系统中的肿瘤转移和细胞介导的免疫 I. 体外肿瘤侵袭性和体内转移形成。
DOI：
10.1002/ijc.2910230215
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发表时间：
1979
影响因子：
6.4
通讯作者：
M. Lohmann‐Matthes
中科院分区：
文献类型：
--
作者：
V. Schirrmacher;G. Shantz;K. Cauer;D. Komitowski;H.‐P. Zimmermannn;M. Lohmann‐Matthes
描述了用于研究肿瘤转移和细胞介导的免疫的同基因模型系统。该系统由两种相关的化学诱导的小鼠淋巴瘤组成，即非转移性亲本细胞系Eb及其转移性变体ESb。纳入一种不相关的化学诱导肿瘤（MDAY）作为特异性对照。血清学分型显示Eb和ESb均为T淋巴源，表达宿主菌株DBA/2的H-2K和H-2D分子。通过各种电子显微镜技术，观察到两种细胞系之间的形态差异。与Eb细胞相比，ESb肿瘤细胞具有更多的多态性核，核膜上有许多迷走神经，细胞表面上的微绒毛表达更突出。肿瘤侵袭性的体外器官培养试验首次在同系小鼠系统中进行，结果显示ESb而不是Eb肿瘤细胞具有附着和侵袭正常组织的能力。因此，ESb肿瘤细胞在体内显示出更高的恶性度。这从它们更高的致瘤性和它们传播和转移以及更快地杀死受体小鼠的能力中显而易见。在对局部原发性肿瘤进行组织学检查时，发现宿主来源的单核细胞（主要是组织细胞）浸润程度存在显著差异。非转移性肿瘤Eb严重浸润，而肿瘤ESb仅含有少量这些细胞。肿瘤细胞系ESb和Eb之间的差异被认为是根据其可能的相关性一般转移。这两个肿瘤的病因进行了讨论，特别是关于它们的相关性。
A syngeneic model system for the study of tumor metastases and cell‐mediated immunity is described. The system consists of two related, chemically induced murine lymphomas, the non‐metastasizing parental line Eb and its metastasizing variant ESb. An unrelated, chemically induced tumor (MDAY) is included for specificity controls. Serological typing revealed that both Eb and ESb were of T lymphoid origin and expressed the H‐2K and H‐2D molecules of the host strain DBA/2. By various electron microscope techniques, morphological differences were observed between the two cell lines. In comparison to Eb cells, ESb tumor cells had a more polymorphic nucleus with many in vagi‐nations of the nuclear envelope and a more prominent expression of microvilli on the cell surface. An in vitro organ culture test for tumor invasiveness, presented here for the first time in a syngeneic murine system, revealed that ESb but not Eb tumor cells had the ability to attach to and invade normal tissue. Accordingly, ESb tumor cells showed higher malignancy in vivo. This was apparent from their higher tumorigenicity and their ability to disseminate and metastasize and to kill recipient mice more quickly. Upon histological examination of the local primary tumors a striking difference was noticed with regard to the degree of infiltration by host‐derived mononuclear cells, mostly histiocytes. The non‐metastasizing tumor Eb was heavily infiltrated while tumor ESb contained only a few of these cells. The differences between the tumor lines ESb and Eb are considered in the light of their possible relevance for metastases in general. The etiology of the two tumors is discussed in particular with respect to their relatedness.