Electrochemically mediated electrodeposition/electropolymerization to yield a glucose microbiosensor with improved characteristics
Electrochemically mediated electrodeposition/electropolymerization to yield a glucose microbiosensor with improved characteristics
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DOI：
10.1021/ac015628m
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发表时间：
2002-01-15
影响因子：
7.4
通讯作者：
Wilson, GS
中科院分区：
文献类型：
--
作者：
Chen, XH;Matsumoto, N;Wilson, GS
描述了一种提供酶的电化学介导沉积和对内源性电活性物质选择性渗透的聚合物层的方法。电沉积法首先用于葡萄糖氧化酶的直接固定，以在Pt电极上产生均匀、薄且致密的膜。然后采用苯酚的电聚合在酶层内形成抗干扰和保护性的多酚膜。此外，由（3-氨丙基）三甲氧基硅烷衍生的稳定性增强膜的构造通过电化学辅助交联。这种位于酶层外的杂化膜有助于提高稳定性和选择透过性。该传感器具有响应时间短（<4s）、灵敏度高（1200 nA/mM）、响应时间短（<4s）、响应时间短（<4s）等特点。cm（2）），内源性电活性物质干扰小，工作寿命大于50天。
A procedure is described that provides for electrochemically mediated deposition of enzyme and a polymer layer permselective for endogenous electroactive species. Electrodeposition was first employed for the direct immobilization of glucose oxidase to produce a uniform, thin, and compact film on a Pt electrode. Electropolymerization of phenol was then employed to form an anti-interference and protective polyphenol film within the enzyme layer. In addition, a stability-reinforcing membrane derived from (3-aminopropyl)trimethoxysilane was constructed by electrochemically assisted cross-linking. This hybrid film outside the enzyme layer contributed to the improved stability and permselectivity. The resulting glucose sensor was characterized by a short response time (< 4 s), high sensitivity (1200 nA/mM(.)cm(2)), low interference from endogenous electroactive species, and working lifetime of more than 50 days.