A subset of DN1p neurons integrates thermosensory inputs to promote wakefulness via CNMa signaling
A subset of DN1p neurons integrates thermosensory inputs to promote wakefulness via CNMa signaling
复制标题
DN1p 神经元的一个子集整合热感觉输入，通过 CNMa 信号传导促进觉醒
DOI：
10.1016/j.cub.2021.02.048
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发表时间：
2021-05-24
期刊：
影响因子：
9.2
通讯作者：
Han, Junhai
中科院分区：
文献类型：
--
作者：
Jin, Xi;Tian, Yao;Han, Junhai
睡眠是一种基本的、进化上保守的行为，它受到许多环境因素的调节。环境温度变化通常发生在气候或季节变化或从高纬度地区到低纬度地区的旅行中，影响动物的生理。提高环境温度调节人类和果蝇的睡眠。虽然已经鉴定了几种温度感觉分子和神经元，但将温度感觉整合到睡眠神经回路中的神经机制仍然知之甚少。在这里，我们揭示了环境温度的长期增加诱导可逆的睡眠减少和受损的睡眠巩固在果蝇通过激活内部热敏前细胞（AC）。AC与后背神经元1（DN 1 p）神经元的子集形成突触接触并释放乙酰胆碱以促进觉醒。此外，我们确定，DN 1 ps的这个子集通过释放CNMa酰胺（CNMa）神经肽，通过CNMa受体抑制Dh 44阳性的大脑间部（PI）神经元，促进觉醒。我们的研究表明，AC-DN 1 p-PI神经回路负责将热敏输入整合到睡眠神经回路中。此外，我们确定CNMa信号通路作为一个新认识的觉醒促进DN 1通路。
Sleep is an essential and evolutionarily conserved behavior that is modulated by many environmental factors. Ambient temperature shifting usually occurs during climatic or seasonal change or travel from high-latitude area to low-latitude area that affects animal physiology. Increasing ambient temperature modulates sleep in both humans and Drosophila. Although several thermosensory molecules and neurons have been identified, the neural mechanisms that integrate temperature sensation into the sleep neural circuit remain poorly understood. Here, we reveal that prolonged increasing of ambient temperature induces a reversible sleep reduction and impaired sleep consolidation in Drosophila via activating the internal thermosensory anterior cells (ACs). ACs form synaptic contacts with a subset of posterior dorsal neuron 1 (DN1p) neurons and release acetylcholine to promote wakefulness. Furthermore, we identify that this subset of DN1ps promotes wakefulness by releasing CNMamide (CNMa) neuropeptides to inhibit the Dh44-positive pars intercerebralis (PI) neurons through CNMa receptors. Our study demonstrates that the AC-DN1p-PI neural circuit is responsible for integrating thermosensory inputs into the sleep neural circuit. Moreover, we identify the CNMa signaling pathway as a newly recognized wakefulness-promoting DN1 pathway.